{"product_id":"biolegend-634102","title":"Biolegend, 634102, Purified anti-Caspase-3 Antibody, 500microl","description":"\u003cp\u003eCaspase 3 is a member of the peptidase family C14. This cytoplasmic caspase functions as a heterotetramer and has two isoforms (one acts as a dominant negative inhibitor). The pro-form of this caspase is approximately 32 kD. Caspase 3 is involved in the caspase activation cascade responsible for apoptosis execution. Caspase 3 is inhibited by c-IAP1, c-IAP2, XIAP, survivin, and Livin and activated by Caspase 6, -8 , and -10, granzyme B, Apaf-1 by cleavage into large p17 and small p12 subunits. Caspase 3 has been shown to cleave PARP, huntingtin, androgen receptor, atrophin-1, DNA- PKcs, D4-GDI, hnRNPs C1\/C2, U1snRNP, PKC delta, ATM, fodrin, gelsolin, ICAD, RIP, X-IAP, STAT1, topoisomerase I, vimentin, Rb, lamin B, mdm2, and β-catenin. The poly6341 antibody has been shown to be useful for western blotting, immunoprecipitation and immunohistochemistry of human Human caspase-3 precursor.\u003cbr\u003e\n500microl\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Polyclonal\u003cbr\u003e\nHost Species: Rabbit\u003cbr\u003e\nImmunogen: Peptide mapping to the amino terminal domain of human caspase-3 prescursor\u003cbr\u003e\nFormulation: This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% gelatin.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C. DO NOT FREEZE.\u003cbr\u003e\nApplication: WB - Quality tested IP - Reported in the literature, not verified in house\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by Western blotting. Western blotting suggested working dilution(s): Use 25µl per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: This product may contain other non-IgG subtypes.\u003cbr\u003e\nAdditional Product Notes: The 125 µL size can be used for approximately 5 Western blots.\u003cbr\u003e\nProduct Citations: Linetsky M, et al. 2020. Free Radic Biol Med. 152:280. PubMed Ramón-Luing LA, et al. 2022. Pathogens. 11:. PubMed\u003cbr\u003e\nRRID: AB_2069874 (BioLegend Cat. No. 634101)\u003cbr\u003e\nStructure: Peptidase family C14, heterotetramer. Two isoforms, pro-Casp3 32 kD\u003cbr\u003e\nInteraction: Active heterodimers between large p17 subunit and small p11 subunit of Casp7 and vice versa. Cleaves PARP, huntingtin, androgen receptor, atrophin-1, DNA- PKcs, D4-GDI, hnRNPs C1\/C2, U1snRNP, PKC delta, ATM, fodrin, gelsolin, ICAD, RIP, X-IAP, STAT1, topo\u003cbr\u003e\nBioactivity: Involved in caspase activation cascade responsible for apoptosis execution. Cleaves\/activates SREBPs, Casp6, Casp7, Casp9\u003cbr\u003e\nBiology Area: Apoptosis\/Tumor Suppressors\/Cell Death, Cell Biology, Neuroscience\u003cbr\u003e\nAntigen References: 1. Goldberg Y, et al. 1996. Nat. Genet. 13:442. 2. Fernandes-Alnemri T, et al. 1996. P. Natl. Acad. Sci. USA 93:7464. 3. Slee E, et al. 2001. J. Biol. Chem. 276:7320. 4. Woo M, et al. 2003. Nat. Immunol. 4:1016.\u003cbr\u003e\nRegulation: Inhibited by dominant negative isoform, c-IAP1, c-IAP2, XIAP, survivin, Livin. Activated when cleaved by Casp6, -8, -10, granzyme B, Apaf-1 into large p17 and small p12 subunits\u003cbr\u003e\nGene ID: 12367\u003cbr\u003e\nUniProt: View information about Caspase-3 on UniProt.org\u003cbr\u003e\nClone: Poly6341\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Caspase 3 preproprotein, Casp3, cpp32, Apopain, Yama, PARP cleavage protease, SREBP cleavage activity 1\u003cbr\u003e\nIsotype: Rabbit Polyclonal IgG\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866471157929,"sku":"634102","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-634102","provider":"Iright","version":"1.0","type":"link"}