{"product_id":"biolegend-636801","title":"Biolegend, 636801, Purified anti-p97\/VCP Antibody, 25μg","description":"\u003cp\u003ep97\/VCP (valosin containing protein) belongs to the AAA ATPase family, predicted molecular weight 97 kD. VCP is associated with a variety of cellular activities, including cell cycle control, membrane fusion after mitosis, regulates spindle disassembly at the end of mitosis, nuclear trafficking and the ubiquitin-proteasome degradation pathway. In many instances, specific adaptor proteins with which VCP forms multiprotein complexes direct its role in a given process. This protein is required for the fusion of ER and Golgi membranes. Clone 4G9 has been shown to be useful for western blotting, immunoprecipitation, and immunofluorescence staining of human p97\/VCP protein.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Human, Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Recombinant human valosin containing protein\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: Upon receipt, store undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: WB - Quality tested IP, ICC - Reported by the developer, not verified in house\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, suggested working dilution(s): Use 5 µg per 5 mL antibody dilution buffer for each mini-gel. For immunofluorescence microscopy: Use a dilution range of 1~4 µg\/mL. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Shchedrina VA, et al. 2011. J Biol Chem. 186:42937. PubMed. Yi L, et al. 2012. Hum Mol Genet. 21:1794. PubMed. Yahiro K, et al. 2012. Infect Immun. 80:1803. PubMed.\u003cbr\u003e\nProduct Citations: Yi L, et al. 2012. Hum Mol Genet. 2.120833333. PubMed Yahiro K, et al. 2012. Infect Immun. 80:1803. PubMed Shchedrina V, et al. 2011. J Biol Chem. 286:42937. PubMed Efimova I, et al. 2021. Front Immunol. 12:643144. PubMed\u003cbr\u003e\nRRID: AB_2257136 (BioLegend Cat. No. 636801) AB_2214641 (BioLegend Cat. No. 636802)\u003cbr\u003e\nStructure: Belongs to the AAA ATPase family, homohexamer, forms a ring-shaped particle of 12.5 nm diameter and displays 6-fold radial symmetry.\u003cbr\u003e\nInteraction: VCIP135, SELS\/VIMP, SYVN1, DERL1, DERL2, DERL3, SVIP\u003cbr\u003e\nBioactivity: VCP is associated with a variety of cellular activities, including cell cycle control, membrane fusion after mitosis, regulates spindle disassembly at the end of mitosis, nuclear trafficking and the ubiquitin-proteasome degradation pathway.\u003cbr\u003e\nBiology Area: Cell Biology, Cell Cycle\/DNA Replication, Neuroscience, Neuroscience Cell Markers\u003cbr\u003e\nMolecular Family: Endoplasmic Reticulum Markers\u003cbr\u003e\nAntigen References: 1. Ye Y, et al. 2001. Nature 414:652.\u003cbr\u003e\nGene ID: 7415\u003cbr\u003e\nUniProt: View information about p97\/VCP on UniProt.org\u003cbr\u003e\nClone: 4G9\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Transitional endoplasmic reticulum ATPase, p97, TERA\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862417756329,"sku":"636801","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-636801","provider":"Iright","version":"1.0","type":"link"}