{"product_id":"biolegend-639958","title":"Biolegend, 639958, Alexa Fluor® 488 anti-CD340 (ErbB2\/HER-2) Antibody, 100μg","description":"\u003cp\u003eCD340, also commonly known as HER-2 or ErbB2, is a member of the epidermal growth factor receptor family of cell membrane tyrosine kinases containing a single transmembrane domain. CD340 contains three furin repeats and two CheY homologous receiver domains in the extracellular region. Although CD340 itself contains no ligand- binding domain, it interacts with other EGF receptor family members to form a heterodimer, stabilize ligand binding, and enhance kinase-mediated downstream signaling. CD340 has been shown to be involved in embryonic development and cancer progression; this protein is amplified in lung adenocarcinoma, some breast cancers, glioblastoma, gastric cancer, and ovarian carcinoma, where it functions as an oncogene. CD340 is a therapeutic target in several human cancers and it has been shown that overexpression in breast cancers confers Taxol resistance.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Recombinant fragment of the intracellular domain of human CD340 (ErbB2\/HER-2)\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.\u003cbr\u003e\nConcentration: 0.5 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: ICFC - Quality tested ICC - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by intracellular flow cytometry using our Cyto-Fast™ Fix\/Perm Buffer Set. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µL volume. For immunocytochemistry, a concentration range of 1.0 - 10.0 μg\/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)\u003cbr\u003e\nAdditional Product Notes: For use in intracellular flow cytometry (ICFC), it is recommended to fix\/permeabilize with Cyto-Fast™ Fix\/Perm Buffer Set (Cat. No. 426803). Fixation\/permeabilization with either True-Phos™ Perm Buffer (Cat. No. 425401) or True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) is not recommended. For use in immunocytochemistry (ICC), it is recommended to fix\/permeabilize with any of the following: -Fixation buffer (Cat. No. 420801) with 0.5 % Triton X-100 -Fixation buffer (Cat. No. 420801) with 100% ice-cold methanol Fixation\/permeabilization with 100% ice-cold methanol alone is not recommended.\u003cbr\u003e\nStructure: CD340 is a 1255 amino acid protein with a predicted molecular weight of 137.9 kD. It is a glycosylated receptor-like transmembrane protein; due to glycosylation, the observed molecular weight by Western Blot is closer to 175-185 kD.\u003cbr\u003e\nDistribution: Cellular membrane and also present in internalized vesicles\u003cbr\u003e\nFunction: Receptor, transcriptional regulator\u003cbr\u003e\nInteraction: Homodimerizes Heterodimerizes with EGFR, ErbB3, ErbB4, PIK3C2B, KPNB1, RANBP2, EEA1, CRM1, CLTC, MUC1, PTK6, RPA194, ACTB, PRKCABP, SRC, MYOC, CPNE3, SORL1, SH3BGRL\u003cbr\u003e\nLigand\/Receptor: Heterodimerizes with other ERBB family members upon ligand binding, but does not directly bind ligand\u003cbr\u003e\nCell Type: Leukemia\u003cbr\u003e\nBiology Area: Cell Biology, Immunology, Synaptic Biology\u003cbr\u003e\nMolecular Family: Adhesion Molecules, CD Molecules\u003cbr\u003e\nAntigen References: Akiyama T, et al. 1986. Science. 232:1644-6. Bargmann CI, et al. 1986. Nature. 319:226-30. Pegram MD, et al. 1997. Oncogene. 15:537-47. Slamon DJ, et al. 1989. Science. 244:707-12. Leone F, et al. 2003. J Leukoc Biol. 74:593-601.\u003cbr\u003e\nGene ID: 2064\u003cbr\u003e\nUniProt: View information about CD340 on UniProt.org\u003cbr\u003e\nClone: W22266D\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Receptor tyrosine-protein kinase ErbB-2 (ERBB2), human epidermal growth factor receptor 2 (HER2), metastatic lymph node gene 19 protein (MLN19), Proto-oncogene Neu, p185(erbB2), cluster of differentiation 340 (CD340), NGL, herstatin\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866436587689,"sku":"639958","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-639958","provider":"Iright","version":"1.0","type":"link"}