{"product_id":"biolegend-640251","title":"Biolegend, 640251, Purified anti-CD73 Antibody, 25μg","description":"\u003cp\u003eCD73 (cluster of differentiation 73) is a membrane-bound glycoprotein and ectoenzyme. To activate adenosine signaling CD73 catalyzes the hydrolysis of AMP generated by CD39 to adenosine. This role makes CD73 crucial to the regulation of purinergic signaling and immune homeostasis and response. CD73 also serves a variety of physiological functions including lymphocyte binding, epithelial ion transport, ischemic preconditioning, tissue-barrier function, T cell activation, and tissue homeostasis. Overexpressed in several cancers and implicated in tumor progression, metastasis, and the impairment of anti-tumor immune response via adenosine signaling, CD73 is a target of interest in cancer therapy. It is also a potential immunotherapy target for the treatment of pain, inflammation, and hypoxia.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Recombinant protein corresponding to the nucleotidase domain of human CD73\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: FC - Quality tested ICC - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.03 µg per million cells in 100 µL volume. For immunocytochemistry, a concentration range of 1.0 - 10.0 μg\/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nAdditional Product Notes: For use in immunocytochemistry (ICC), it is recommended to fix\/permeabilize with the following: -Fixation buffer (Cat. No. 420801) with 0.5 % Triton X-100 -Fixation buffer (Cat. No. 420801) with 100% ice-cold methanol -100% ice-cold methanol only This product is not recommended for use in immunohistochemistry in formalin fixed, paraffin-embedded tissues (IHC-P) or Western blot (WB).\u003cbr\u003e\nRRID: AB_3717149 (BioLegend Cat. No. 640251) AB_3717149 (BioLegend Cat. No. 640252)\u003cbr\u003e\nStructure: CD73 is a 574 amino acid protein with a predicted molecular weight of 63 kD.\u003cbr\u003e\nDistribution: Cell membrane\u003cbr\u003e\nFunction: Hydrolase, metabolic protein, CD marker, enzyme\u003cbr\u003e\nInteraction: AP-2, SMAD, SP-1, CD39, GSDMD\u003cbr\u003e\nLigand\/Receptor: Metal-binding, nucleotide-binding, zinc\u003cbr\u003e\nCell Type: Lymphocytes, T cells\u003cbr\u003e\nBiology Area: Cell Biology\u003cbr\u003e\nMolecular Family: CD Molecules, Enzymes and Regulators, Innate Immune Signaling\u003cbr\u003e\nAntigen References: Knapp K, et al. 2012. Structure. 20:2161. Minor M, et al. 2019. Am. J. Physiol. Cell Physiol. 317:C1079. Yu M, et al. 2020. Nat. Commun. 11:515.\u003cbr\u003e\nGene ID: 4907\u003cbr\u003e\nUniProt: View information about CD73 on UniProt.org\u003cbr\u003e\nClone: W23165D\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: clulster of differentiation 73 (CD73), 5'-Nucleotidase Ecto (NT5E), CALJA, ENT, NT5, EN, E5NT\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866438783145,"sku":"640251","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-640251","provider":"Iright","version":"1.0","type":"link"}