{"product_id":"biolegend-640654","title":"Biolegend, 640654, Ultra-LEAF™ Purified anti-human BMP12\/GDF-7 Antibody, 1mg","description":"\u003cp\u003eBMP12 is a member of the TGF-β superfamily that includes TGF-β proteins, activins\/inhibins, morphogenetic proteins (BMPs), and GDFs. The TGF-β superfamily plays a key role in skeletal development, bone formation, and stem cell differentiation. BMPs signal through heterotetrameric complex receptors, which include four type I receptors (ALK-1, ALK-2, ALK-3, and ALK-6) and three type II receptors (BMPRII, ActRIIA, and ActRIIB). BMP12, BMP13, and BMP14 share 82% amino acid sequence homology and also share similar function. These proteins induce neotendon\/ligament formation in vivo after implanted in ectopic sites. Thus, administration of subcutaneous or intramuscular BMP12, BMP13, and BMP14 activate the tenogenic pathway in human and rat bone marrow derived mesenchymal stem cells (MSCs). BMP12 induces the proliferation of tendon fibroblasts in vitro and the expression of procollagen Type I and Type III. Also, topical administration of ASC and BMP12 promote cell accumulation and matrix remodeling in a model of tendon repair. In addition, this combined administration induces an M2 macrophage phenotype that enriches tendon healing.\u003cbr\u003e\n1mg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Recombinant Human BMP12\/GDF-7\u003cbr\u003e\nFormulation: 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.\u003cbr\u003e\nEndotoxin Level: Less than 0.01 EU\/μg of the protein (\u0026lt; 0.001 ng\/μg of the protein) as determined by the LAL test\u003cbr\u003e\nPreparation: The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: The antibody is bottled at the concentration indicated on the vial, typically between 2 mg\/mL and 3 mg\/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.\u003cbr\u003e\nApplication: Neut - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by neutralizing the ALP activity induced by 4 μg\/mL of Recombinant Human BMP12\/GDF-7 in ATDC-5 cells. ND50 range: 3 – 18 μg\/mL.\u003cbr\u003e\nRRID: AB_3717170 (BioLegend Cat. No. 640653) AB_3717170 (BioLegend Cat. No. 640654)\u003cbr\u003e\nStructure: Dimer\u003cbr\u003e\nDistribution: Tenoblast, mesenchymal cells, pericytes in the endotenon, tendon fibroblasts\u003cbr\u003e\nFunction: BMP12 promotes cell growth and stem cell tenogenic differentiation in bone marrow MSCs. Key role in the development of interneurons, sensory neurons, and seminal vesicles.\u003cbr\u003e\nInteraction: Bone marrow mesenchymal stem cells (MSCs), adipose derived MSCs, neurons\u003cbr\u003e\nLigand\/Receptor: Type I (BMPR-IB) and type II (BMPR-II or Activin RII)\u003cbr\u003e\nCell Type: Mesenchymal cells, Neurons\u003cbr\u003e\nBiology Area: Neuroscience\u003cbr\u003e\nMolecular Family: Growth Factors\u003cbr\u003e\nAntigen References: Lou J, et al. 2001. J Orthop Res. 6:1199-202. Fu SC, et al. 2003. Life Sci. 72:2965-74. Mazerbourg S, et al. 2005. J Biol. Chem. 280: 32122-32. Lo L, et al. 2005. Proc Natl Acad Sci U S A. 102:7192-97. Eliasson P, et al. 2008. Clin Orthop Relat Res. 466:1592-97. Lee JY, et al. 2011. PLoS One. 6:e17531. Gelberman RH, et al. 2017. Clin Orthop Relat Res. 475:2318-331.\u003cbr\u003e\nGene ID: 151449\u003cbr\u003e\nUniProt: View information about BMP12\/ GDF-7 on UniProt.org\u003cbr\u003e\nClone: W23031A\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Growth Differentiation Factor 7, GDF7, GDF-7, BMP-12\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003cbr\u003e\nQ: Do you guarantee that your antibodies are totally pathogen free?\u003cbr\u003e\nA: BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.\u003cbr\u003e\nQ: Does BioLegend test each Ultra-LEAF™ antibody by functional assay?\u003cbr\u003e\nA: No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.\u003cbr\u003e\nQ: Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?\u003cbr\u003e\nA: No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.\u003cbr\u003e\nQ: Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?\u003cbr\u003e\nA: We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866437243049,"sku":"640654","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-640654","provider":"Iright","version":"1.0","type":"link"}