{"product_id":"biolegend-643801","title":"Biolegend, 643801, Purified anti-β-actin Antibody, 25μg","description":"\u003cp\u003eβ-actin is a ubiquitously expressed and highly conserved 42 kD cytoplasmic protein involved in cell motility. This critical cytoskeletal component can be disrupted by drugs such as cytochalasin. Because β-actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection (such as Western blotting).\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Human, Mouse, Rat\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: WB - Quality tested ICC, IHC-P - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 µL per 5 mL antibody dilution buffer for each mini-gel. For immunocytochemistry, a concentration range of 1.0 - 5.0 µg\/mL is recommended. For immunohistochemistry, a concentration range of 5 - 10 µg\/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: The binding of this antibody to its target is sensitive to salt concentration. For consistent results, please use TBS\/T buffer for Western blotting that contains 0.15 M NaCl as indicated in the BioLegend recommended protocol.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Lawson BR, et al. 2007. J. Immunol. 178:5366. Joyce CW, et al. 2006. J. Biol. Chem. 281:33053. Yanagiya T,et al.2007.Obesity.15:572. KishidaT,et al. 2007.J. Immunol. 179:8554. Ouimet M, et al. 2008. Arterisocler Thromb Vasc Biol. 28:1144. Toltlt LJ,et al. 2008.J. Immunol. 181:2165. Sawada. T,et al. 2008. J. Biol. Chem. 283:26820. Ikeda D, et al. 2008. Endocrinology. 149:6037. Ikeda Y, et al. 2010. Mol Endocrinol. 24:1338. PubMed Kaieda S, et al. 2010. J. Biol Chem. 285:21478. PubMed Cambos M, et al. 2011. J. Leukoc. Biol. 89:157. PubMed\u003cbr\u003e\nProduct Citations: Fathi M, et al. 2023. iScience. 26:106482. PubMed Tetikoglu S, et al. 2023. Chem Biodivers. 20:e202300401. PubMed Xu Y, et al. 2022. Nat Commun. 13:980. PubMed Herr SA, et al. 2022. Neural Regen Res. 17:1505. PubMed Greenberg D, et al. 2015. Infect Immun . 83: 4277 - 4292. PubMed Sugimoto C, et al. 2022. Elife. 11:. PubMed Kaya-Sezginer E, et al. 2022. Andrology. 10:1441. PubMed Sims S, et al. 2021. Adv Virol. 2021:5569844. PubMed Callahan SM, et al. 2021. Curr Protoc. 1:e294. PubMed Callahan S, et al. 2020. Cell Microbiol. 22:e13210. PubMed Hadjicharalambous MR, et al. 2019. Sci Rep. 9:6020. PubMed Kaieda S, et al. 2010. J Biol Chem. 285:21478. PubMed Rodríguez E, et al. 2022. Commun Biol. 5:41. PubMed Bruggen R, et al. 2015. PLoS One. 10: 0144555. PubMed Budida R, et al. 2017. Eur J Immunol. 47:1819. PubMed Hsiao CC, et al. 2021. Cells. 10:. PubMed Scorza M 2011. J Leukoc Biol. 89:159. PubMed Lovatt M, et al. 2018. Antioxidants (Basel). 0.416666667. PubMed Lai CC, et al. 2022. Biomed Rep. 16:19. PubMed Kakaradov B, et al. 2017. Nat Immunol. 18:422. PubMed Nagatake T, et al. 2018. J Allergy Clin Immunol. 142:470. PubMed Ikeda Y, et al. 2010. Mol Endocrinol. 24:1338. PubMed Wagner AK, et al. 2017. Nat Commun. 8:15627. PubMed Qin S, et al. 2018. Oncol Lett. 128:1283. PubMed Lalor R, et al. 2019. Nutrients. 11. PubMed\u003cbr\u003e\nRRID: AB_2223201 (BioLegend Cat. No. 643801) AB_2223199 (BioLegend Cat. No. 643802)\u003cbr\u003e\nStructure: Actin, cytoplasmic 1\u003cbr\u003e\nDistribution: Ubiquitously expressed in the cytoplasm of all eukaryotic cells\u003cbr\u003e\nFunction: Actins are highly conserved proteins that are involved in cell motility\u003cbr\u003e\nBiology Area: Cell Biology, Neuroscience, Neuroscience Cell Markers\u003cbr\u003e\nMolecular Family: Microfilaments\u003cbr\u003e\nAntigen References: 1. Hanukoglu I, et al. 1983. J. Mol. Biol. 163:673. 2. Nakajima-Iijima S, et al. 1985. Proc. Natl. Acad. Sci. 82:6133. 3. Ponte P, et al. 1984. Nucleic Acids Res. 12:1687.\u003cbr\u003e\nGene ID: 11461\u003cbr\u003e\nUniProt: View information about beta-actin on UniProt.org\u003cbr\u003e\nClone: 2F1-1\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Actin, cytoplasmic 1\u003cbr\u003e\nIsotype: Mouse IgG2b, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862322172073,"sku":"643801","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-643801","provider":"Iright","version":"1.0","type":"link"}