{"product_id":"biolegend-644817","title":"Biolegend, 644817, Brilliant Violet 605™ anti-T-bet Antibody, 125microl","description":"\u003cp\u003eT-bet, also known as T-box transcription factor T-bet, is considered to be a \"master regulator\" of Th1 lymphoid development controlling the production of the cytokine IFN-γ. T-bet is widely expressed in hematopoietic cells including stem cells, NK cells, B cells, and T cells. T-bet is critical for the control of microbial pathogens, and knockout animals show multiple physiologic and inflammatory features characteristic of asthma. T-bet expression is optimally observed after IL-12 stimulation and can be suppressed by addition of the Th2 cytokine IL-4 or neutralization of IL-12.\u003cbr\u003e\n125microl\u003cbr\u003e\nVerified Reactivity: Human, Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 605™ under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: ICFC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining using our True-Nuclear™ Transcription Factor Staining Protocol. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.Brilliant Violet 605™ excites at 405 nm and emits at 603 nm. The bandpass filter 610\/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 605™ is a trademark of Sirigen Group Ltd.Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.\u003cbr\u003e\nExcitation Laser: Violet Laser (405 nm)\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation2 and immunofluorescence microscopy3.NOTE: For flow cytometric staining with this clone, True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended over the Foxp3 Fix\/Perm Buffer Set (Cat. No. 421403) and the Nuclear Factor Fixation and Permeabilization Buffer Set (Cat. No. 422601).\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Szabo SJ, et al. 2000. Cell 100:655. (ICFC, WB) Hwang ES, et al. 2005. J. Exp. Med. 202:1289. (ICFC, WB, IP) Neurath MF, et al. 2002. J. Exp. Med. 195:1129. (IF) Hsieh CY, et al. 2012. J Pharmacol Exp. 343:125. PubMed.\u003cbr\u003e\nProduct Citations: Prota G, et al. 2015. Vaccine. 410: 01290-6. PubMed Ogishi M, et al. 2023. J Exp Med. 220: . PubMed Whitehead GS, et al. 2022. JCI Insight. 7:. PubMed Leeansyah E, et al. 2015. PLoS Pathog. 11: 1005072. PubMed Pack AD, et al. 2021. Cell Reports. 36:109586. PubMed Haertel E, et al. 2018. Eur J Immunol. 48:1001. PubMed Witkowski M, et al. 2021. Nature. 600:295. PubMed Tauriainen J, et al. 2017. Sci Rep. 7:40354. PubMed Glaubitz J, et al. 2022. Nat Commun. 13:4502. PubMed Prosser A, et al. 2021. Cell Reports. 35(7):109141. PubMed Toubal A, et al. 2020. Nat Commun. 3755:11. PubMed Kotov DI, et al. 2018. J Immunol. 200:2004. PubMed Eccles JD, et al. 2020. Cell Rep. 30:351. PubMed Becker W, et al. 2021. J Crohns Colitis. 15:1032. PubMed Zeis P, et al. 2020. Immunity. 53:775. PubMed Meijgaarden K, et al. 2015. PLoS Pathog. 11:1004671. PubMed de Bourayne M, et al. 2017. J Leukoc Biol. 101:703. PubMed Pantelidou C, et al. 2022. NPJ Breast Cancer. 8:102. PubMed Prosser A, et al. 2021. STAR Protoc. 2:100810. PubMed Bennstein SB, et al. 2020. eLife. 9:e55232.. PubMed Cobbold SP, et al. 2018. Front Immunol. 9:1381. PubMed\u003cbr\u003e\nRRID: AB_11219388 (BioLegend Cat. No. 644817)\u003cbr\u003e\nStructure: T-box transcription factor, approximately 58 kD.\u003cbr\u003e\nDistribution: Nuclear; expressed in T cells, hematopoietic stem cells, NK cells, B cells, lung, spleen.\u003cbr\u003e\nFunction: Th1-specific T-box transcription factor controlling expression of the hallmark Th1 cytokine, interferon gamma (IFN-γ). T-bet expression is critical for the control of microbial pathogens.\u003cbr\u003e\nCell Type: B cells, Hematopoietic stem and progenitors, NK cells, T cells, Tregs\u003cbr\u003e\nBiology Area: Cell Biology, Immunology, Transcription Factors\u003cbr\u003e\nMolecular Family: Nuclear Markers\u003cbr\u003e\nAntigen References: 1. Szabo SJ, et al. 2000. Cell 100:655. 2. Szabo SJ, et al. 2002. Science 295:338. 3. Finotto S, et al. 2002. Science 295:336. 4. Mullen AC, et al. 2001. Science 292:1907.\u003cbr\u003e\nGene ID: 30009\u003cbr\u003e\nUniProt: View information about T-bet on UniProt.org\u003cbr\u003e\nClone: 4B10\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: T-box expressed in T cells, T box 21, TBLYM\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862408908969,"sku":"644817","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-644817","provider":"Iright","version":"1.0","type":"link"}