{"product_id":"biolegend-646151","title":"Biolegend, 646151, Purified anti-Pan AKT Antibody, 25μg","description":"\u003cp\u003eThe AKT family of proteins, also known as Protein Kinase B (PKB), consists of three isoforms: AKT1, AKT2, and AKT3. These serine\/threonine kinases are pivotal in regulating various cellular processes, including glucose metabolism, apoptosis, cell proliferation, transcription, and cell migration. AKT1 is primarily involved in cellular survival pathways and is a key player in inhibiting apoptosis, promoting cell growth, and protein synthesis. AKT2 is crucial in the insulin signaling pathway, facilitating glucose transport and maintaining glucose homeostasis. AKT3 is predominantly expressed in the brain and is essential for brain development. Dysregulation of AKT signaling is associated with numerous diseases, including cancer, diabetes, and neurological disorders such as Alzheimer's disease. Overexpression or mutations in AKT genes can lead to uncontrolled cell growth and survival, contributing to tumorigenesis in various cancers. Additionally, aberrant AKT signaling is implicated in insulin resistance and type 2 diabetes, as well as neurodegenerative diseases.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Human, Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Synthetic peptide for human AKT\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: WB - Quality tested ICC, IHC-P, ICFC - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg\/mL. For immunocytochemistry, a concentration range of 1.25 - 10.0 μg\/mL is recommended. For immunohistochemistry, a concentration range of 2.5 - 10.0 µg\/mL is suggested. For flow cytometric staining, the suggested use of this reagent is ≤ 0.06 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: This antibody detects all 3 isoforms of AKT protein kinases (AKT1, AKT2, AKT3).\u003cbr\u003e\nAdditional Product Notes: For use of this antibody in intracellular flow cytometry (ICFC), we recommend fixation and permeabilization using either True-Phos™ Perm Buffer (Cat. No. 425401), or Cyto-Fast Fix\/Perm Buffer set (Cat. No. 426803) For use of this antibody in Immunocytochemistry (ICC), we recommend fixation\/permeabilization using either PFA-Triton X-100, or Methanol only. For use of this antibody in immunohistochemistry on formalin-fixed paraffin-embedded tissues (IHC-P), we recommend antigen retrieval using Citrate Buffer (Cat. No. 420902), or Tris-EDTA pH 9.0 Antigen Retrieval Buffer (Cat. No. 422704).\u003cbr\u003e\nRRID: AB_3699072 (BioLegend Cat. No. 646151) AB_3699072 (BioLegend Cat. No. 646152)\u003cbr\u003e\nStructure: AKT Molecular Weight: ~ 56 kDa\u003cbr\u003e\nDistribution: Nucleoplasm and cytoplasm\u003cbr\u003e\nBiology Area: Apoptosis\/Tumor Suppressors\/Cell Death, Cancer Biomarkers, Cell Biology, Cell Motility\/Cytoskeleton\/Structure, Cell Proliferation and Viability, Immuno-Oncology\u003cbr\u003e\nMolecular Family: Phospho-Proteins, Protein Kinases\/Phosphatase\u003cbr\u003e\nAntigen References: Krzeslak A, et al. 2011. Endocrine Pathology. 22:206. Jian K, et al. 2003. Blood. 101:236. Manning BD, et al. 2017. Cell. 169:381. Song M, et al. 2019. Cancer Res. 79:1019. Revathidevi S, et al. 2019. Semin Cancer Biol. 59:80.\u003cbr\u003e\nGene ID: 2072081000\u003cbr\u003e\nUniProt: View information about AKT on UniProt.org\u003cbr\u003e\nClone: A21001A\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Protein kinase B, PKB, RAC-Alpha, RAC-alpha serine\/threonine-protein kinase, RAC-beta serine\/threonine-protein kinase, RAC-gamma serine\/threonine-protein kinase\u003cbr\u003e\nIsotype: Mouse IgG2b, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866072567977,"sku":"646151","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-646151","provider":"Iright","version":"1.0","type":"link"}