{"product_id":"biolegend-647201","title":"Biolegend, 647201, Purified anti-Caspase-11 Antibody, 25μg","description":"\u003cp\u003eMurine caspase-11 is an upstream protease of caspase-1 and interacts with the proform of caspase-1 which in turn activates IL-1 beta. Caspase-11 is not constitutively expressed in most cell types. Its expression can be upregulated by LPS, IFN-gamma, and other pro-inflammatory stimuli. NF-κB and STAT1 were reported to be involved in the activation. Caspase-11 knock -out mice were resistant to endotoxin shock and do not produce IL-1 after stimulation with LPS. Their production of IFN-gamma was also deficient.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: His tagged bacterial fusion protein of p 30 of caspase 11\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: WB - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): 1 to 2 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Schmidt RL, et al. 2012. PLoS One. 7:e45186. PubMed.\u003cbr\u003e\nProduct Citations: Khameneh H, et al. 2011. PLoS One. 6:e23025. PubMed Schmidt R, Lenz L 2012. PLoS One. 7:e45186. PubMed Min L, et al. 2012. J Immunol. 188:1789. PubMed Evavold CL, et al. 2021. Cell. 184(17):4495-4511.e19. PubMed\u003cbr\u003e\nRRID: AB_1937283 (BioLegend Cat. No. 647201) AB_1937281 (BioLegend Cat. No. 647202)\u003cbr\u003e\nStructure: This murine caspase 11 has pro-enzymes a 38 and 43 kD. The activated form consists of p30 and p10.\u003cbr\u003e\nDistribution: Not constitutively expressed in most cell types, but can be induced by different pro-inflmmatory stimuli including LPS.\u003cbr\u003e\nFunction: Essential for the activation of caspase-1, thus involved in the maturation of IL-1beta and IL-18. Both are important mediators of inflammation.\u003cbr\u003e\nInteraction: Caspase-1\u003cbr\u003e\nBiology Area: Apoptosis\/Tumor Suppressors\/Cell Death, Cell Biology, Neuroscience\u003cbr\u003e\nAntigen References: 1. Lee J et al. 2001. J. Biol. Chem. 276:329562. Schauvliege R et al. 2002. J. Biol. Chem. 277:416243. Wang S et al. 1998. Cell 92:501\u003cbr\u003e\nGene ID: 201161\u003cbr\u003e\nUniProt: View information about Caspase-11 on UniProt.org\u003cbr\u003e\nClone: Cas11.17D9\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Casp11\u003cbr\u003e\nIsotype: Rat IgG2a\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862961934505,"sku":"647201","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-647201","provider":"Iright","version":"1.0","type":"link"}