{"product_id":"biolegend-648805","title":"Biolegend, 648805, Purified anti-SMC3 Antibody, 25μg","description":"\u003cp\u003eSMC3 (Structural Maintenance of Chromosomes 3) is a core component of the cohesin complex, a group of proteins essential for chromosome cohesion, segregation, DNA repair, and transcriptional regulation in eukaryotic cells. SMC3 belongs to the Structural Maintenance of Chromosomes (SMC) family of ATPases, which includes other members such as SMC1, SMC2, SMC4, SMC5, and SMC6. Together with SMC1A, RAD21 (SCC1), and STAG proteins (SA1\/SA2), SMC3 forms the ring-shaped cohesin complex that topologically encircles sister chromatids, holding them together after DNA replication until their separation during mitosis or meiosis. SMC3 and the broader cohesin complex are involved in regulating gene expression through chromatin architecture remodeling. Cohesin interacts with CTCF (CCCTC-binding factor), a key architectural protein, to form chromatin loops and topologically associating domains (TADs). These structures bring enhancers and promoters into close proximity, modulating transcriptional programs necessary for development and differentiation.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Human, Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Recombinant protein fragment of human SMC3\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: WB - Quality tested ICC - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg\/mL. For immunocytochemistry, a concentration range of 2.5 - 10.0 μg\/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nAdditional Product Notes: For use in immunocytochemistry (ICC), it is recommended to fix\/permeabilize with either of the following: -Fixation buffer (Cat. No. 420801) with 0.5 % Triton X-100 -Fixation buffer (Cat. No. 420801) with 100% ice-cold methanol\u003cbr\u003e\nRRID: AB_3717174 (BioLegend Cat. No. 648805) AB_3717174 (BioLegend Cat. No. 648806)\u003cbr\u003e\nStructure: SMC3 is a 1217 amino acid protein with a predicted molecular weight of 141.5 kD.\u003cbr\u003e\nDistribution: Located in nucleus\u003cbr\u003e\nFunction: Forms the ring-shaped cohesin complex\u003cbr\u003e\nInteraction: Forms a heterodimer with SMC1A or SMC1B in cohesin complexes; interacts with RAD21 and STAG proteins\u003cbr\u003e\nBiology Area: Cell Biology, Cell Cycle\/DNA Replication\u003cbr\u003e\nAntigen References: Deardorff MA, et al. 2007. Am. J. Hum. Genet. 80:485-94. Beckouët F, et al. 2010. Mol. Cell. 39:689-99. Gil-Rodríguez MC, et al. 2015. Hum. Mutat. 36:454-62. Wang D, et al. 2018. Biomed. Pharmacother.104:788-797.\u003cbr\u003e\nGene ID: 9126\u003cbr\u003e\nUniProt: View information about SMC3 on UniProt.org\u003cbr\u003e\nClone: W23212B\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Structural maintenance of chromosomes protein 3, SMC protein 3, SMC-3, Basement membrane-associated chondroitin proteoglycan (Bamacan), BAM, BMH, Chondroitin sulfate proteoglycan 6, CSPG6, Chromosome-associated polypeptide, hCAP\u003cbr\u003e\nIsotype: Rat IgG2b, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866439307433,"sku":"648805","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-648805","provider":"Iright","version":"1.0","type":"link"}