{"product_id":"biolegend-648807","title":"Biolegend, 648807, Purified anti-Bcl-XL Antibody, 25μg","description":"\u003cp\u003eBcl-X, a member of the Bcl-2 family of proteins, is encoded by the BCL2L1 gene and plays a crucial role in regulating apoptosis. Bcl-X exists in two main isoforms: Bcl-XL, which is anti-apoptotic, and Bcl-XS, which is pro-apoptotic. The balance between these isoforms is regulated through alternative splicing, influenced by various splice factors, transcription factors, and signaling pathways. Dysregulation of Bcl-X splicing has been implicated in several diseases, particularly cancer and diabetes. In cancer, overexpression of Bcl-XL can lead to resistance to chemotherapy, while in diabetes, increased Bcl-XS expression can result in apoptosis of pancreatic β-cells.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Recombinant fragment of human Bcl-X protein\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: WB - Quality tested ICC, ICFC - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.125 - 1.0 µg\/mL. For immunohistochemistry, a concentration range of 1.0 - 10.0 µg\/mL is suggested. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: Due to homology, this antibody may detect the isoform Bcl-XS (18.8 kDa) which is a result of alternative splicing. However, in Western Blot, this isoform was not detected. The canonical sequence, isoform Bcl-XL, was detected by this antibody in Western Blot.\u003cbr\u003e\nAdditional Product Notes: For use of this antibody in intracellular flow cytometry (ICFC), we recommend fixation and permeabilization either using True-Nuclear™ Transcription Factor Buffer set (Cat. No. 424401) or Cyto-Fast™ Fix-Perm Buffer set (Cat. No. 426803). We DO NOT recommend using True-Phos™ Perm Buffer (Cat. No. 425401). For use of this antibody in immunohistochemistry on formalin-fixed paraffin-embedded tissues (IHC-P), we recommend antigen retrieval using either Citrate Buffer (Cat. No. 420902), or Tris-EDTA pH 9.0 Antigen Retrieval Buffer (Cat. No. 422704).\u003cbr\u003e\nRRID: AB_3717173 (BioLegend Cat. No. 648807) AB_3717173 (BioLegend Cat. No. 648808)\u003cbr\u003e\nStructure: Bcl-XL is a 233 amino acid length protein with a molecular weight of approximately 26 kDa.\u003cbr\u003e\nDistribution: Mitochondrial membrane and cytoplasm\u003cbr\u003e\nInteraction: Bcl-XL and Bcl-XS interact with: Bad, Bak, Bim, Bid, Bax and p53\u003cbr\u003e\nBiology Area: Apoptosis\/Tumor Suppressors\/Cell Death, Cancer Biomarkers, Cell Biology, Cell Death, Cell Proliferation and Viability\u003cbr\u003e\nMolecular Family: Adaptor Proteins, Mitochondrial Markers, Scaffold Proteins\u003cbr\u003e\nAntigen References: Dou Z, et al. 2021. J Exp Clin Cancer Res. 40, 194. Bharatham N, et al. 2011. PLOS One. doi.org\/10.1371\/journal.pone.0026014. Stevens M, et al. 2019. Front Genet.10:doi.org\/10.3389\/fgene.2019.00804. Findley HW, et al. 1997. Blood. 89:2986.\u003cbr\u003e\nGene ID: 598\u003cbr\u003e\nUniProt: View information about Bcl-XL on UniProt.org\u003cbr\u003e\nClone: W23168D\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: BCLX, BCL-X, Bcl-2-like protein 1, BCL2L1, Bcl2-L-1, Apoptosis regulator Bcl-X, BCL2L\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866439176361,"sku":"648807","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-648807","provider":"Iright","version":"1.0","type":"link"}