{"product_id":"biolegend-648815","title":"Biolegend, 648815, Purified anti-Keratin 14 Antibody, 100μg","description":"\u003cp\u003eKeratin 14 is a member of the keratin family, the most diverse group of intermediate filaments. This gene product, a type I keratin, is usually found as a heterotetramer with two keratin 5 molecules, a type II keratin. Together they form the cytoskeleton of epithelial cells. Mutations in the genes for these keratins are associated with epidermolysis bullosa simplex.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Recombinant peptide corresponding to the C terminus of human keratin 14\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: IHC-P - Quality tested ICC, WB, ICFC - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 10.0 µg\/mL is suggested. For immunocytochemistry, a concentration range of 1.0 - 10.0 μg\/mL is recommended. For western blotting, the suggested use of this reagent is 0.1 - 1.0 µg\/mL. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nAdditional Product Notes: For use in immunohistochemistry on formalin-fixed paraffin-embedded tissue (IHC-P), it is recommended to perform antigen retrieval using either Citrate Buffer, 10X (Cat. No. 420902) or Tris-EDTA pH 9.0 Antigen Retrieval Buffer (10X) (Cat. No. 422704). For use in immunocytochemistry (ICC), it is recommended to fix\/permeabilize with any of the following: -Fixation buffer (Cat. No. 420801) with 0.5 % Triton X-100 -Fixation buffer (Cat. No. 420801) with 100% ice-cold methanol -100% ice-cold methanol only For use in intracellular flow cytometry (ICFC), it is recommended to fix\/permeabilize with any of the following: -Cyto-Fast™ Fix\/Perm Buffer Set (Cat. No. 426803) -True-Phos™ Perm Buffer (Cat. No. 425401) -True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) -True-Nuclear™ Transcription Factor Buffer Set gives the most robust signal. This product does not cross-react with mouse keratin 14 by Western Blot.\u003cbr\u003e\nRRID: AB_3717183 (BioLegend Cat. No. 648814) AB_3717183 (BioLegend Cat. No. 648815)\u003cbr\u003e\nStructure: Keratin 14 is a 472 amino acid protein with a molecular mass of 52 kD.\u003cbr\u003e\nDistribution: Tissue distribution: skin Cellular distribution: cytoplasm, nucleus\u003cbr\u003e\nFunction: Nonhelical tail domain is involved in promoting KRT5-KRT14 filaments to self-organize into large bundles, enhances the mechanical properties involved in resilience of keratin intermediate filaments in vitro.\u003cbr\u003e\nInteraction: Keratin 5, desmosomal proteins, plectin\u003cbr\u003e\nBiology Area: Cell Biology, Cell Motility\/Cytoskeleton\/Structure, Neuroscience, Neuroscience Cell Markers\u003cbr\u003e\nMolecular Family: Intermediate Filaments\u003cbr\u003e\nAntigen References: Wend P, et al. 2013. EMBO J. 32:1977-89. Welm AL, et al. 2005. Proc Natl Acad Sci USA. 102:4324-9 Hu Y, et al. 2001. Nature. 410:710-714. Yuspa SH, et al. 1989. J Cell Biol. 109:1207-1217. Roop DR, et al. 1984. J Biol Chem. 259:8037-8040.\u003cbr\u003e\nGene ID: 3861\u003cbr\u003e\nUniProt: View information about Keratin 14 on UniProt.org\u003cbr\u003e\nClone: W24068C\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Keratin, type I cytoskeletal 14, CK-14, cytokeratin 14, keratin 14 (epidermolysis bullosa simplex, Dowling-Meara, Koebner), KRT, KRT14\u003cbr\u003e\nIsotype: Rat IgG2b, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866440224937,"sku":"648815","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-648815","provider":"Iright","version":"1.0","type":"link"}