{"product_id":"biolegend-648837","title":"Biolegend, 648837, Purified anti-HMGA2 Antibody, 100μg","description":"\u003cp\u003eHMGA2, also known as the high-mobility group AT-hook 2, is a nonhistone transcriptional regulator belonging to the high mobility group (HMG) protein family with AT-hook DNA binding motif. HMGA2 is highly expressed during early mammalian embryogenesis but is hardly detected in normal adult tissues. Recently, it was discovered that HMGA2 is upregulated in a variety of tumors including gastric cancer, ovarian cancer, non-small cell lung cancer and colorectal cancer, suggesting that it has a role in controlling tumor progression. The cancers with overexpression HMGA2 have a significantly stronger response to double strand breaks in DNA caused by radiation therapy and certain chemotherapy treatments. HMGA2 also has been implicated in diet-induced obesity in the gene knockout study of the mouse.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Human, Mouse, Rat\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Recombinant human HMGA2 protein\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: WB - Quality tested ICC, IHC-P - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.125 - 1.0 µg\/mL. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg\/mL is recommended. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0 - 10.0 µg\/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nAdditional Product Notes: The clone cross-reacts with mouse and rat in western blot (WB). For immunocytochemistry (ICC), we recommend 4% PFA fixation (Cat. No. 420801) followed by permeabilization with Triton X-100 or ice-cold methanol. For immunohistochemistry on FFPE tissues (IHC-P), antigen retrieval with Citrate Buffer (Cat. No. 420901) or Tris-EDTA pH 9.0 (Cat. No. 422704) are recommended.\u003cbr\u003e\nRRID: AB_3720546 (BioLegend Cat. No. 648836) AB_3720546 (BioLegend Cat. No. 648837)\u003cbr\u003e\nStructure: HMGA2 is a 109 amino acid protein with a predicted molecular weight of 12 kD.\u003cbr\u003e\nDistribution: Nucleus. Expressed predominantly during embryogenesis.\u003cbr\u003e\nFunction: Transcriptional regulator, cell cycle regulation.\u003cbr\u003e\nInteraction: E4F1, NEK2\u003cbr\u003e\nCell Type: Embryonic Stem Cells, Hematopoietic stem and progenitors\u003cbr\u003e\nBiology Area: Cell Biology, Cell Cycle\/DNA Replication, Cell Proliferation and Viability, Stem Cells\u003cbr\u003e\nAntigen References: Cleynen I, et al. 2008. Int J Oncol. 32:289. Monzen K, et al. 2008. Nat Cell Biol. 10:567. Fusco A and Fedele M. 2007. Nat Rev Cancer. 7:899. Rawlinson N.J, et al. 2008. Cancer Genet Cytogenet.181(2):119. Wei J.J, et al. 2010. Am J Surg Pathol. 34(1):18. Mahajan,A, et al. 2010. Mod Pathol. 23(5),673. Ming- Boo L, et al. 2005. Cancer Res. 65(15):6622. Zhou X, et al. 1995. Nature. 376(6543):771. Anand A, et al. 2000. Nature Genetics. 24:377.\u003cbr\u003e\nGene ID: 8091\u003cbr\u003e\nUniProt: View information about HMGA2 on UniProt.org\u003cbr\u003e\nClone: W23012E\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: High Mobility Group AT-Hook 2, High-Mobility Group (Nonhistone Chromosomal) Protein\u003cbr\u003e\nIsotype: Rat IgG2b, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866437046441,"sku":"648837","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-648837","provider":"Iright","version":"1.0","type":"link"}