{"product_id":"biolegend-650051","title":"Biolegend, 650051, Purified anti-Claudin-6 Antibody, 25μg","description":"\u003cp\u003eClaudin-6 is a member of the claudin family of proteins, which consists of at least 24 members. Claudin proteins are integral membrane proteins characterized by 4 transmembrane regions and 2 extracellular loops that form tight junctions. Abnormal expression can disrupt tight junction integrity, playing an important role in the occurrence and development of cancers. Claudin-6 in particular, has been considered a therapeutic target because it is commonly expressed in tumor tissue but not in healthy adult tissue.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Recombinant fragment of human Claudin-6\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: WB - Quality tested IHC-P, ICC - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.125 - 1 µg\/mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 10 - 1 ug\/mL is suggested. For immunocytochemistry, a concentration range of 10 - 1 μg\/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: For use of this antibody in Immunocytochemistry (ICC), we recommend fixation\/permeabilization using PFA-Triton X-100 and Methanol. We do not recommend PFA-Methanol fixation\/permeabilization. For use of this antibody in immunohistochemistry on formalin-fixed paraffin-embedded tissues (IHC-P), we recommend antigen retrieval using Citrate Buffer, 10X (Cat. No. 420902), or Tris-EDTA buffer.\u003cbr\u003e\nRRID: AB_3683486 (BioLegend Cat. No. 650051) AB_3683486 (BioLegend Cat. No. 650052)\u003cbr\u003e\nStructure: Consists of four transmembrane domains and two extracellular loops. It is a 220 amino acid length protein with a predicted molecular weight of 23 kDa.\u003cbr\u003e\nDistribution: Cellular membrane\u003cbr\u003e\nFunction: Tight junctions and cell adhesion\u003cbr\u003e\nAntigen References: Reinhard K, et al. 2020. Science. 367(6476):446-453. Huan D, et al. 2021. Mol Med Rep. 24(3):677. Ushiku T, et al. 2012. Histophatology. 61(6):1043-56. Gowrikumar S, et al. 2019. Int J Mol Sci. 21:53.\u003cbr\u003e\nGene ID: 9074\u003cbr\u003e\nUniProt: View information about Claudin-6 on UniProt.org\u003cbr\u003e\nClone: W22275B\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Claudin-6, Claudin 6, CLDN6, Skullin\u003cbr\u003e\nIsotype: Rat IgG2a, λ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866436882601,"sku":"650051","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-650051","provider":"Iright","version":"1.0","type":"link"}