{"product_id":"biolegend-651011","title":"Biolegend, 651011, Direct-Blot™ HRP anti-STAT3 Phospho (Tyr705) Antibody, 100microl","description":"\u003cp\u003eTyrosine phosphorylation of STAT3 at Tyr705 occurs in response to LIF, IL-6, leptin, OSM, EGF, PDGF, and HGF. It plays a key role in cell growth and apoptosis through mediating expression of a variety of genes in response to the stimuli.\u003cbr\u003e\n100microl\u003cbr\u003e\nVerified Reactivity: Human, Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: KLH conjugated modified synthetic peptide\u003cbr\u003e\nFormulation: This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.\u003cbr\u003e\nApplication: WB - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested dilution is 1:1000-1:4000. The optimal dilution should be determined by titration for each individual assay of interest. 25 µl and 100 µl of Direct-Blot™ HRP antibody can be used for approximately 5 and 20 Western blots, respectively, at the recommended concentration\/dilution.\u003cbr\u003e\nApplication Notes: The STAT3 Phospho (Tyr705) antibody recognizes the regulatory tyrosine phosphorylation of human STAT3 protein and has been shown to be useful for Western blotting.\u003cbr\u003e\nProduct Citations: Aung Naing et al. 2018. Cancer cell. 34(5):775-791 . PubMed\u003cbr\u003e\nRRID: AB_2616951 (BioLegend Cat. No. 651011)\u003cbr\u003e\nStructure: STAT3 is a 770 amino acid protein of 88 kD. It consists of a DNA binding domain, a SH2 domain, a regulatory tyrosine responsible for binding of SH2 domain, and a C-terminal transactivation domain.\u003cbr\u003e\nDistribution: Ubiquitous.\u003cbr\u003e\nFunction: STAT3 is tyrosine phosphorylated by receptor kinases in response to a variety of cytokines and growth factors. It forms homo- or heterodimer with STAT1 when tyrosine is phosphorylated, and then translocates to nucleus, acting as a transcription regulator. It is also essential for the differentiation of TH17 cells, which is involved in autoimmune diseases.\u003cbr\u003e\nCell Sources: Cytoplasm. Translocate to nucleus in response to tyrosine phosphorylation.\u003cbr\u003e\nCell Type: Embryonic Stem Cells, Neural Stem Cells\u003cbr\u003e\nBiology Area: Cell Biology, Neuroscience, Neuroscience Cell Markers, Signal Transduction, Stem Cells, Synaptic Biology, Transcription Factors\u003cbr\u003e\nMolecular Family: Phospho-Proteins\u003cbr\u003e\nAntigen References: 1. Akira S, et al. 1994. Cell 77:63. 2. Zhang X, et al. 1995. Science 267:1990. 3. Sanchez-Margalet V, et al. 2001. Cell. Immunol. 211:30. 4. Simon A, et al. 2000. Science 290:144. 5. Hoey T, et al. 1999. Adv. Immunol. 71:145.\u003cbr\u003e\nRegulation: The small GTPase Rac1 binds and regulates activity of STAT3.\u003cbr\u003e\nGene ID: 6774\u003cbr\u003e\nUniProt: View information about STAT3 Phospho Tyr705 on UniProt.org\u003cbr\u003e\nClone: 13A3-1\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Signal transducer and activator of transcription 3, Acute-phase response factor, APRF, HIES\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46860801015977,"sku":"651011","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-651011","provider":"Iright","version":"1.0","type":"link"}