{"product_id":"biolegend-652427","title":"Biolegend, 652427, PE\/Dazzle™ 594 anti-mouse Ki-67 Antibody, 25μg","description":"\u003cp\u003eThe nuclear protein Ki-67 was first identified by the monoclonal antibody Ki-67, which was generated by immunizing mice with nuclei of the L428 Hodgkin lymphoma cell line. Ki-67 protein plays an essential role in ribosomal RNA transcription and cell proliferation. Expression of Ki-67 occurs during G1, S, G2, and M phase, while in G0 phase the Ki-67 protein is not detectable. Ki-67 is strongly expressed in proliferating cells and has been reported as a prognostic marker in various tumors.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: E. coli expressed partial mouse Ki-67 recombinant protein, 1816-2163 aa.\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with PE\/Dazzle™ 594 under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: ICFC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by our Ki-67 staining protocol below. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Medina-Reyes EI, et al. 2015. Environ Res. 136:424. PubMed Guillaumond F, et al. 2015. PNAS. 112:2473. PubMed Sharma SK, et al. 2015. J Immunol. 194:5529. PubMed Rodero MP, et al. 2014. J. Invest. Dermatol. 7:1991-7. PubMed\u003cbr\u003e\nProduct Citations: Duraiswamy J, et al. 2021. Cancer Cell. 39:1623. PubMed Chen X, et al. 2022. J Extracell Vesicles. 11:e12279. PubMed Lin YH 2023. Immunity. 56(1):207-223.e8. PubMed Wang C, et al. 2021. Cell Rep. 37:110021. PubMed Bayik D, et al. 2020. Cancer Discov. 1.256944444. PubMed Montalban-Arques A, et al. 2021. Cell Host Microbe. :. PubMed\u003cbr\u003e\nRRID: AB_2632695 (BioLegend Cat. No. 652427) AB_2632696 (BioLegend Cat. No. 652428)\u003cbr\u003e\nStructure: 325 kD protein containing a forkhead-associated domain (FHA) and 13 tandem repeats\u003cbr\u003e\nDistribution: Nucleus and chromosome\u003cbr\u003e\nFunction: Required for cell cycle progression and proliferation\u003cbr\u003e\nInteraction: Interacts with KIF15; binds to MKI67IP through FHA domain\u003cbr\u003e\nBiology Area: Cell Biology, Cell Cycle\/DNA Replication, Transcription Factors\u003cbr\u003e\nMolecular Family: Nuclear Markers\u003cbr\u003e\nAntigen References: 1. Starborg M, et al. 1996. J. Cell. Sci. 109:143. 2. Byeon IJ, et al. 2005. Nat. Struct. Mol. Biol. 12:987. 3. Yerushalmi R, et al. 2010. Lancet. Oncol. 11:174. 4. Beltrami AP, et al. 2001. N. Engl. J. Med. 344:1750. 5. Sachsenberg N, et al. 1998. J. Exp. Med. 187:1295. 6. Nagy Z, et al. 1997. Acta. Neuropathol. 93:294.\u003cbr\u003e\nGene ID: 17345\u003cbr\u003e\nUniProt: View information about Ki-67 on UniProt.org\u003cbr\u003e\nClone: 16A8\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: KIA, proliferation-related Ki-67 antigen\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46864352706729,"sku":"652427","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-652427","provider":"Iright","version":"1.0","type":"link"}