{"product_id":"biolegend-654003","title":"Biolegend, 654003, Alexa Fluor® 647 anti-IDO1 Antibody, 25μg","description":"\u003cp\u003eIDO1 is also known as Indolamine 2,3-dioxygenase, Indole 2,3-dioxygenase, and Indoleamine-pyrrole 2,3-dioxygenase. IDO is a ubiquitously expressed cytoplasmic protein with a predicted molecular weight of approximately 45 kD. Indoleamine 2,3-dioxygenase (IDO) is one the best known IFN-γ inducible genes. The product of IDO gene catalyzes the degradation of the essential amino acid L-tryptophan to N-formylkynurenine. IDO has been implicated to protect against intracellular and extracellular pathogens. It also has been shown to maintain the special immune suppressive status of immune-privileged sites such as the brain, eyes, kidney, and placenta.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Recombinant mouse IDO1.\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: ICFC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm \/ 635 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses\u003cbr\u003e\nExcitation Laser: Red Laser (633 nm)\u003cbr\u003e\nApplication Notes: Clone 2E2\/IDO1 reacts with mouse IDO1. It does not appear to react with mouse IDO2. Clone 2E2\/IDO1 is the same clone as anti-IDO1 clone 2E2.6.\u003cbr\u003e\nProduct Citations: Michela Miani et al. 2018. Cell metabolism. 28(4):557-572 . PubMed Mansouri S, et al. 2020. Mucosal Immunol. 0.954861111. PubMed Chen C, et al. 2020. Front Cell Neurosci. 14:8. PubMed Molina MS, et al. 2021. Front Immunol. 12:699128. PubMed Li A, et al. 2018. Cancer Lett. 431:54. PubMed\u003cbr\u003e\nRRID: AB_2564584 (BioLegend Cat. No. 654003) AB_2564585 (BioLegend Cat. No. 654004)\u003cbr\u003e\nStructure: 403 amino acids with a predicted molecular weight of 45 kD.\u003cbr\u003e\nDistribution: Cytoplasm\u003cbr\u003e\nFunction: Indoleamine 2,3-dioxygenase (IDO) is an IFN-γ inducible gene. It catalyzes the degradation of the essential amino acid L-tryptophan to N-formylkynurenine. IDO has been implicated to protect against intracellular and extracellular pathogens.\u003cbr\u003e\nInteraction: This enzyme acts on multiple tryptophan substrates including D-tryptophan, L-tryptophan, 5-hydroxy-tryptophan, tryptamine, and serotonin.\u003cbr\u003e\nBiology Area: Cell Biology, Immunology\u003cbr\u003e\nAntigen References: 1. Habara-Ohkubo A, et al. 1991. Gene 105:221. 2. Munn DH, et al. 2002. Science 297:1867. 3. Frumento G, et al. 2002. J. Exp. Med. 196:459. 4. Muller AJ, et al. 2005. Nature Med. 11:312. 5. Lob S, et al. 2009. Nature Rev. Cancer 9:445.\u003cbr\u003e\nGene ID: 15930\u003cbr\u003e\nUniProt: View information about IDO1 on UniProt.org\u003cbr\u003e\nClone: 2E2\/IDO1\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Indolamine 2,3-dioxygenase, Indole 2,3-dioxygenase, Indoleamine-pyrrole 2,3-dioxygenase\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862319943849,"sku":"654003","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-654003","provider":"Iright","version":"1.0","type":"link"}