{"product_id":"biolegend-655202","title":"Biolegend, 655202, Purified anti-TCF1 (TCF7) Antibody, 100μg","description":"\u003cp\u003eTCF1 is the first identified member of the T-cell-specific transcription factor family.  It plays an important role in T cell development and differentiation. TCF1 is inactivated by association with the transcriptional repressor TLE proteins. During Wnt signaling, the transcriptional coactivator CTNNB1 accumulates and, in turn, replaces the transcriptional repressor associated with TCF1. Interaction with CTNNB1 results in transactivation of TCF1 target genes. Deletion of TCF1 causes massive apoptosis of double positive thymocyte, suggesting that TCF1 is required for thymocyte survival during T cell development. In addition to its function in thymus, TCF1 promotes T cell differentiation to Th2 cells in the periphery through transcriptional activation of GATA3.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Partial TCF1 recombinant protein (116-334 aa)\u003cbr\u003e\nFormulation: This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: Affinity purified\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: Upon receipt, store undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: WB - Quality tested ICC, IHC-P - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 0.5 µg per mL. For immunocytochemmistry microscopy, a concentration range of 1-5 µg\/mL is recommended. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1 - 10 µg\/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nProduct Citations: Duraiswamy J, et al. 2021. Cancer Cell. 39:1623. PubMed Poon MML, et al. 2023. Nat Immunol. 24:309. PubMed Sekine T, et al. 2020. Sci Immunol. 5: . PubMed Tian Y, et al. 2019. Cell Rep. 29:4482. PubMed Liu F, et al. 2020. J Immunother Cancer. 8:. PubMed O'Boyle KC, et al. 2020. Methods Mol Biol. 2111:1. PubMed Cobb DA, et al. 2022. J Immunother Cancer. 10:. PubMed Bengsch B et al. 2018. Immunity. 48(5):1029-1045 . PubMed\u003cbr\u003e\nRRID: AB_2562103 (BioLegend Cat. No. 655202)\u003cbr\u003e\nStructure: 384 amino acids, predicted molecular weight of 42 kD. Contains a HMG box DNA binding domain and a CTNNB1 (β-catenin) binding domain.\u003cbr\u003e\nDistribution: Nucleus\u003cbr\u003e\nFunction: TCF1 is a transcription factor, involved in the canonical Wingless\/Integration 1 (Wnt) signaling pathway. TCF1 is essential for survival of CD4+CD8+ double positive thymocytes and differentiation of T cells in the periphery.\u003cbr\u003e\nInteraction: TCF1 interacts with CTNNB1, TLE1, TLE2, TLE3, TLE4, and AES.\u003cbr\u003e\nCell Type: Tregs\u003cbr\u003e\nBiology Area: Cell Biology, Immunology, Transcription Factors\u003cbr\u003e\nMolecular Family: Nuclear Markers, TCRs\u003cbr\u003e\nAntigen References: 1. Mao CD, et al. 2011. Crit. Rev. Eukaryot. Gene Expr. 21:207. 2. Germar K, et al. 2011. P. Natl. Acad. Sci. USA 108:20060. 3. Wang R, et al. 2011. J. Immunol. 187:5964. 4. Weber BN, et al. 2011. Nature 476:63. 5. Jeannet G, et al. 2010. P. Natl. Acad. Sci. USA 107:9777. 6. Staal FJ, et al. 1999. Int. Immunol. 11:317.\u003cbr\u003e\nGene ID: 6932\u003cbr\u003e\nUniProt: View information about TCF1 on UniProt.org\u003cbr\u003e\nClone: 7F11A10\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: T-cell-specific transcription factor 1, transcription factor 7, TCF7\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46864378626217,"sku":"655202","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-655202","provider":"Iright","version":"1.0","type":"link"}