{"product_id":"biolegend-656111","title":"Biolegend, 656111, Pacific Blue™ anti-SOX2 Antibody, 25tests","description":"\u003cp\u003eSOX2 is the most studied member of SRY-related box transcription factor family. It binds to target genes through its highly conserved HMG box domain. Inactivation of the SOX2 gene causes lethality during embryonic development. SOX2 knockdown in embryonic stem cells results in their differentiation. Co-expression of SOX with OCT4, MYC, and KLF4 is sufficient to reprogram somatic cells to induce pluripotent stem cells (iPSCs), which exert similar characteristics as natural pluripotent stem cells. These findings indicate that SOX2 is crucial for the self-renewal and pluripotency of embryonic stem cells. In addition, over-expression of SOX2 has been found in various types of malignant cancer. Knockdown of SOX2 results in cell cycle arrest by downregulating cyclin D1 and inhibition of tumor cell proliferation, suggesting that SOX2 is involved in activating genes associated with tumor progression.\u003cbr\u003e\n25tests\u003cbr\u003e\nVerified Reactivity: Human, Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Full length human SOX recombinant protein\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with Pacific Blue™ under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: ICFC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Nuclear™ Transcription Factor Staining Protocol. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. * Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses\u003cbr\u003e\nExcitation Laser: Violet Laser (405 nm)\u003cbr\u003e\nApplication Notes: NOTE: For flow cytometric staining with this clone, True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended. This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing).\u003cbr\u003e\nProduct Citations: Kendirli A, et al. 2023. Nat Neurosci. 1713:26. PubMed Wideman SK, et al. 2023. PLoS Pathog. e1011679:19. PubMed Bellon A, et al. 2019. Front Mol Neurosci. 11:323. PubMed\u003cbr\u003e\nRRID: AB_2566188 (BioLegend Cat. No. 656111) AB_2566189 (BioLegend Cat. No. 656112)\u003cbr\u003e\nStructure: 317 amino acids, predicted molecular weight of 34 kD, contains a HMG box domain responsible for DNA binding\u003cbr\u003e\nDistribution: Nucleus\u003cbr\u003e\nFunction: Transcription factor that regulates the expression of the genes involved in embryonic development\u003cbr\u003e\nInteraction: Interacts with FGFR1, SOX3, and ZSCAN10\u003cbr\u003e\nCell Type: Embryonic Stem Cells, Mesenchymal Stem Cells, Neural Stem Cells\u003cbr\u003e\nBiology Area: Cell Biology, Cell Cycle\/DNA Replication, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells, Transcription Factors\u003cbr\u003e\nAntigen References: 1. Rizzino A. 2009. Wiley Interdiscip. Rev. Syst. Biol. Med. 1:228. 2. Stolzenburg S, et al. 2012. Nucleic Acids Res. 40:6725. 3. Lai YS, et al. 2012. Proc. Natl. Acad. Sci. USA. 109:3772. 4. Jeong CH, et al. 2010. Stem Cells 28:2141. 5. Xiang R, et al. 2011. Br. J. Cancer 104:1410. 6. Card DA, et al. 2008. Mol. Cell Biol. 28:6426.\u003cbr\u003e\nGene ID: 6657\u003cbr\u003e\nUniProt: View information about SOX2 on UniProt.org\u003cbr\u003e\nClone: 14A6A34\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: SRY-related HMG-box gene 2, SRY (sex determining region Y)-box 2, MCOPS3, ANOP3\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46864329670825,"sku":"656111","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-656111","provider":"Iright","version":"1.0","type":"link"}