{"product_id":"biolegend-656808","title":"Biolegend, 656808, Brilliant Violet 421™ anti-Nestin Antibody, 100μg","description":"\u003cp\u003eNestin is a class VI intermediate filament protein originally found in neuronal stem cells, and is required for survival and renewal of neural progenitor cells. In mixtures, Nestin forms heterodimers with vimentin or internexin, participating in the remodeling of the cell. Nestin was found to be expressed on vascular endothelial progenitor cells and various neoplasms. It is proposed to be a novel angiogenesis marker and cancer therapeutic target.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Fusion protein\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: ICC - Quality testedIHC-P - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 0.1 - 10 μg\/ml is recommended. For immunohistochemical staining on formalin-fixed paraffin-embedded tissue sections, the suggested use of this reagent is 5.0 - 10 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application. Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450\/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd. Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.\u003cbr\u003e\nExcitation Laser: Violet Laser (405 nm)\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Jozef S, et al. 2012. Biomed. Pap. Med. Fac. 156:135. (IHC)\u003cbr\u003e\nProduct Citations: Rocktäschel P, et al. 2019. Epilepsy Behav. 101:106581. PubMed Tamai K, et al. 2022. Cell Rep Methods. 2:100314. PubMed\u003cbr\u003e\nRRID: AB_2566634 (BioLegend Cat. No. 656808)\u003cbr\u003e\nStructure: 1621 amino acids with predicted molecular weight of 177 kD. The observed molecular weight is larger than 250 kD.\u003cbr\u003e\nDistribution: Cytosol\u003cbr\u003e\nFunction: Required for survival and renewal of neural progenitor cells; plays roles in angiogenesis and tumor formation\u003cbr\u003e\nInteraction: Vimentin, alpha-internexin and FHOD3\u003cbr\u003e\nCell Type: Neural Stem Cells\u003cbr\u003e\nBiology Area: Angiogenesis, Cell Biology, Cell Motility\/Cytoskeleton\/Structure, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells\u003cbr\u003e\nMolecular Family: Intermediate Filaments\u003cbr\u003e\nAntigen References: Matsuda Y, et al. 2013. World J. Gastroenterol. 19:42. Matsuda Y, et al. 2012. Med. Mol. Morphol. 45:59. Krupkova O Jr, et al. 2010. Neoplasma. 57:291. Hombach-Klonisch S, et al. 2008. Arch. Immunol. Ther. Exp. (Warsz). 56:165. Pilkington GJ, et al. 2005. Cell Prolif. 38:423.\u003cbr\u003e\nGene ID: 10763\u003cbr\u003e\nUniProt: View information about Nestin on UniProt.org\u003cbr\u003e\nClone: 10C2\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: NES\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003cbr\u003e\nQ: What is the F\/P ratio range of our BV421™ format antibody reagents?\u003cbr\u003e\nA: It is lot-specific. On average it ranges between 2-4.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46860778733737,"sku":"656808","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-656808","provider":"Iright","version":"1.0","type":"link"}