{"product_id":"biolegend-656810","title":"Biolegend, 656810, Alexa Fluor® 647 anti-Nestin Antibody, 100μg","description":"\u003cp\u003eNestin is a class VI intermediate filament protein originally found in neuronal stem cells, and is required for survival and renewal of neural progenitor cells. In mixtures, Nestin forms heterodimers with vimentin or internexin, participating in the remodeling of the cell. Nestin was found to be expressed on vascular endothelial progenitor cells and various neoplasms. It is proposed to be a novel angiogenesis marker and cancer therapeutic target.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Fusion protein\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: ICC - Quality testedIHC-P - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 2.5 - 5.0 μg\/ml is recommended. For immunohistochemical staining on formalin-fixed paraffin-embedded tissue sections, a concentration range of 5.0 - 10.0 µg\/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm \/ 635 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses\u003cbr\u003e\nExcitation Laser: Red Laser (633 nm)\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Jozef S, et al. 2012. Biomed. Pap. Med. Fac. 156:135. (IHC)\u003cbr\u003e\nProduct Citations: Woloshuk A, et al. 2021. Cytometry A. 99:707. PubMed\u003cbr\u003e\nRRID: AB_2616772 (BioLegend Cat. No. 656810)\u003cbr\u003e\nStructure: 1621 amino acids with predicted molecular weight of 177 kD. The observed molecular weight is larger than 250 kD.\u003cbr\u003e\nDistribution: Cytosol\u003cbr\u003e\nFunction: Required for survival and renewal of neural progenitor cells; plays roles in angiogenesis and tumor formation\u003cbr\u003e\nInteraction: Vimentin, alpha-internexin and FHOD3\u003cbr\u003e\nCell Type: Neural Stem Cells\u003cbr\u003e\nBiology Area: Angiogenesis, Cell Biology, Cell Motility\/Cytoskeleton\/Structure, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells\u003cbr\u003e\nMolecular Family: Intermediate Filaments\u003cbr\u003e\nAntigen References: Matsuda Y, et al. 2013. World J. Gastroenterol. 19:42. Matsuda Y, et al. 2012. Med. Mol. Morphol. 45:59. Krupkova O Jr, et al. 2010. Neoplasma. 57:291. Hombach-Klonisch S, et al. 2008. Arch. Immunol. Ther. Exp. (Warsz). 56:165. Pilkington GJ, et al. 2005. Cell Prolif. 38:423.\u003cbr\u003e\nGene ID: 10763\u003cbr\u003e\nUniProt: View information about Nestin on UniProt.org\u003cbr\u003e\nClone: 10C2\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: NES\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46860778373289,"sku":"656810","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-656810","provider":"Iright","version":"1.0","type":"link"}