{"product_id":"biolegend-657052","title":"Biolegend, 657052, Purified anti-c-Fos Antibody, 100μg","description":"\u003cp\u003eThe c-Fos protein, or FBJ murine viral (v-Fos) oncogene homolog, is a member of the Fos family of transcription factors. c-Fos forms heterodimers with proteins of the Jun family of transcription factors to form the AP-1 (Activator Protein-1) complex, which regulates critical cellular processes, including cell proliferation, differentiation, and survival. C-Fos has been demonstrated to play a critical role in angiogenesis, bone development, and in the progression of cancer. C-Fos is also often used as an indirect marker of neuronal activity.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Human, Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Recombinant fragment of human c-Fos\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: WB - Quality tested ICC, ICFC - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg\/mL. For immunocytochemistry, a concentration range of 1.25 - 10.0 μg\/mL is recommended. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nAdditional Product Notes: For use in immunocytochemistry (ICC), it is recommended to fix\/permeabilize with either of the following: - Fixation Buffer (Cat. No. 420801) with 0.5 % Triton X-100 - Fixation Buffer (Cat. No. 420801) with 100% ice-cold methanol For use in intracellular flow cytometry (ICFC), it is recommended to fixation\/permeabilization with True-Phos™ Perm Buffer (Cat. No. 425401).\u003cbr\u003e\nRRID: AB_3717128 (BioLegend Cat. No. 657051) AB_3717128 (BioLegend Cat. No. 657052)\u003cbr\u003e\nStructure: c-Fos is a 380 amino acid protein with a predicted molecular weight of 41 kD.\u003cbr\u003e\nDistribution: Nucleus\u003cbr\u003e\nFunction: FOS and JUN oncoproteins form dimeric complexes that stimulate transcription of genes containing AP-1 regulatory elements. FOS was the first transcription factor identified to have a critical function in the development of cells required for skeletal formation.\u003cbr\u003e\nInteraction: Interacts with a variety of proteins including c-Jun, TFIID, NCOA2, NFAT1, CHOP, ELK1, SRC1, and ETS1\u003cbr\u003e\nBiology Area: Apoptosis\/Tumor Suppressors\/Cell Death, Cancer Biomarkers, Cell Biology, Cell Cycle\/DNA Replication, Cell Death, Cell Proliferation and Viability, Transcription Factors\u003cbr\u003e\nAntigen References: Dony C and Gruss P. 1987. Nature. 328:711-4. Grigoriadis AE, et al. 1994. Science. 266:443-8. Glover JN and Harrison SC. 1995. Nature. 373:257-61.\u003cbr\u003e\nGene ID: 2353\u003cbr\u003e\nUniProt: View information about c-Fos on UniProt.org\u003cbr\u003e\nClone: W23257D\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Oncogene Fos, FBJ murine viral (v-fos) oncogene homolog, G0\/G1 Switch Regulatory Protein 7, Activator Protein 1, GOS7, cfos\u003cbr\u003e\nIsotype: Rat IgG2b, λ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866439569577,"sku":"657052","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-657052","provider":"Iright","version":"1.0","type":"link"}