{"product_id":"biolegend-657802","title":"Biolegend, 657802, Purified anti-Lin28A Antibody, 100μg","description":"\u003cp\u003eLIN28A is a highly conserved RNA-binding protein and reprogramming factor that plays important roles in development, self-renewal of stem cells, glucose metabolism and tumorigenesis. Lin28A modulates not only miRNAs, particularly the let-7 family miRNAs, but also the translation of mRNAs that are important for cell growth and metabolism. LIN28A\/B has been observed to be overexpressed in a wide range of solid tumors and hematological malignancies, and has emerged as a new therapeutic target candidate for cancer.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Recombinant human Lin28A from E. coli\u003cbr\u003e\nFormulation: This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: Upon receipt, store undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: WB - Quality tested ICC, ICFC - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.025 - 1.0 µg per ml (1:500 - 1:20,000 dilution). For immunocytochemistry, the suggested use of this reagent is 1.0 - 5.0 µg per ml. For flow cytometric staining, the suggested use of this reagent is 0.06 - 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: HepG2 and HEL cells were selected for specificity testing because they express LIN28B mRNA but lack detectable LIN28A expression (source: Human Protein Atlas). This clone is specific for Lin28A does not recognize Lin28B.\u003cbr\u003e\nRRID: AB_2562650 (BioLegend Cat. No. 657802)\u003cbr\u003e\nStructure: 209 amino acids, predicted molecular weight of 28 kD with the characteristic of a cold shock domain (CSD) and two tandem Cys-Cys-His-Cys (CCHC)-type zinc-binding motifs (CCHCx2)\u003cbr\u003e\nDistribution: Cytoplasm and nucleus; localizes to cytoplasmic processing bodies and stress granules\u003cbr\u003e\nFunction: Development, self-renewal of stem cells, glucose metabolism and tumorigenesis; regulates Let-7 family miRNA, translation of mRNA\u003cbr\u003e\nInteraction: EIF3S2, NCL and ZCCHC11\/TUT4\u003cbr\u003e\nCell Type: Embryonic Stem Cells\u003cbr\u003e\nBiology Area: Apoptosis\/Tumor Suppressors\/Cell Death, Cell Biology, Cell Cycle\/DNA Replication, Chromatin Remodeling\/Epigenetics, Immunology, Protein Synthesis, Signal Transduction, Stem Cells\u003cbr\u003e\nAntigen References: 1. Shyh-Chang N, et al. 2013. Cell Stem Cell. 12:395. 2. Thornton JE, et al. 2012. Trends Cell Biol. 22:474. 3. Huang Y. 2012. Wiley Interdiscip. Rev. RNA. 3:483. 4. Cho J, et al. 2012. Cell. 151:765. 5. Li N, et al. 2012. J. Biol. Chem. 287:17386. 6. Zhu H, et al. 2011. Cell 147:81.\u003cbr\u003e\nGene ID: 79727\u003cbr\u003e\nUniProt: View information about Lin28A on UniProt.org\u003cbr\u003e\nClone: 14E6-4E6\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Protein Lin-28 Homolog A,Lin-28A, CSDD1,ZCCHC1, Zinc Finger CCHC Domain-Containing Protein 1\u003cbr\u003e\nIsotype: Mouse IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46860806652073,"sku":"657802","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-657802","provider":"Iright","version":"1.0","type":"link"}