{"product_id":"biolegend-657807","title":"Biolegend, 657807, Alexa Fluor® 488 anti-Lin28A Antibody, 25tests","description":"\u003cp\u003eLIN28A is a highly conserved RNA-binding protein and reprogramming factor that plays important roles in development, self-renewal of stem cells, glucose metabolism and tumorigenesis. Lin28A modulates not only miRNAs, particularly the let-7 family miRNAs, but also the translation of mRNAs that are important for cell growth and metabolism. LIN28A\/B has been observed to be overexpressed in a wide range of solid tumors and hematological malignancies, and has emerged as a new therapeutic target candidate for cancer.\u003cbr\u003e\n25tests\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Recombinant human Lin28A from E. coli\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: ICFC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)\u003cbr\u003e\nApplication Notes: This clone is specific for Lin28A does not recognize Lin28B.\u003cbr\u003e\nRRID: AB_2924623 (BioLegend Cat. No. 657807) AB_2924623 (BioLegend Cat. No. 657808)\u003cbr\u003e\nStructure: 209 amino acids, predicted molecular weight of 28 kD with the characteristic of a cold shock domain (CSD) and two tandem Cys-Cys-His-Cys (CCHC)-type zinc-binding motifs (CCHCx2)\u003cbr\u003e\nDistribution: Cytoplasm and nucleus; localizes to cytoplasmic processing bodies and stress granules\u003cbr\u003e\nFunction: Development, self-renewal of stem cells, glucose metabolism and tumorigenesis; regulates Let-7 family miRNA, translation of mRNA\u003cbr\u003e\nInteraction: EIF3S2, NCL and ZCCHC11\/TUT4\u003cbr\u003e\nCell Type: Embryonic Stem Cells\u003cbr\u003e\nBiology Area: Apoptosis\/Tumor Suppressors\/Cell Death, Cell Biology, Cell Cycle\/DNA Replication, Chromatin Remodeling\/Epigenetics, Immunology, Protein Synthesis, Signal Transduction, Stem Cells\u003cbr\u003e\nAntigen References: Shyh-Chang N, et al. 2013. Cell Stem Cell. 12:395-406. Thornton JE, et al. 2012. Trends Cell Biol. 22:474-82. Huang Y. 2012. Wiley Interdiscip Rev RNA. 3:483-94. Cho J, et al. 2012. Cell. 151:765-777. Li N, et al. 2012. J Biol Chem. 287:17386-17397. Zhu H, et al. 2011. Cell 147:81-94.\u003cbr\u003e\nGene ID: 79727\u003cbr\u003e\nUniProt: View information about Lin28A on UniProt.org\u003cbr\u003e\nClone: 14E6-4E6\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Protein Lin-28 Homolog A, Lin-28A, CSDD1, ZCCHC1, Zinc Finger CCHC Domain-Containing Protein 1\u003cbr\u003e\nIsotype: Mouse IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46860806455465,"sku":"657807","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-657807","provider":"Iright","version":"1.0","type":"link"}