{"product_id":"biolegend-659702","title":"Biolegend, 659702, Purified anti-NLRC4 (IPAF) Antibody, 100μg","description":"\u003cp\u003eNLRC4 , also known as IPAF, is a member of the NOD-like receptor (NLR) family. The NLR proteins share a conserved DNA-binding NACHT domain, a caspase recruitment domain (CARD), and a leucine-rich repeat (LRR) domain responsible for pathogen pattern recognition. NLRC4 was found to play an important role in the innate immune response against bacterial infection. During Gram-negative bacteria infections, such as Shigella, Salmonella, Legionella, or Pseudomonas, NLRC4 detects and is activated by translocated bacterial flagellin or PrgJ-like rod proteins subsequently resulting in the assembly of the NLRC4 inflammasome. The activated inflammasome drives proteolytic activation of caspase-1, leading to the processing and secretion of the proinflamatory cytokines IL-1β and IL-18.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Partial human NLRC4 recombinant protein (1-88 aa)\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: WB - Quality tested ICC - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.5 µg per mL. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nProduct Citations: Baker GJ, et al. 2016. Oncoimmunology. e1163461:5. PubMed Fytianos K, et al. 2017. ACS Nano. 375:11. PubMed\u003cbr\u003e\nRRID: AB_2563347 (BioLegend Cat. No. 659702)\u003cbr\u003e\nStructure: 1024 amino acids, predicted molecular weight of 116 kD. Contains a CARD domain, a NACHT domain, and 14 leucine-rich repeats.\u003cbr\u003e\nDistribution: Cytoplasm.\u003cbr\u003e\nFunction: NLRC4 is involved in the assembly of the inflammasome complex, activation of pro-caspase-1, and induction of matured proinflammatory cytokines during bacterial infections.\u003cbr\u003e\nInteraction: Foms homo-oligomer in NLRC4 inflammasome. Interacts with Caspase-1, ASC, NOD2, NALP1, and BCL10.\u003cbr\u003e\nCell Type: Dendritic cells\u003cbr\u003e\nBiology Area: Cell Biology, Immunology, Innate Immunity, Signal Transduction\u003cbr\u003e\nAntigen References: 1. Cai S, et al. 2012. J. Immunol. 188:5623. 2. Franchi L, et al. 2012. Nat. Immunol. 13:449. 3. Carvalho FA, et al. 2012. Mucosal Immunol. 5:288. 4. Tomalka J, et al. 2011. PLoS Pathog. 7:e1002379. 5. Pereira MS, et al. 2011. J. Immunol. 187:6447. 6. Sauer JD, et al. 2011. Proc. Natl. Acad. Sci. USA. 108:12419.\u003cbr\u003e\nGene ID: 58484\u003cbr\u003e\nUniProt: View information about NLRC4 on UniProt.org\u003cbr\u003e\nClone: 6H9B13\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: NLR family CARD domain-containing protein 4, CARD, LRR, and NACHT-containing protein (CLAN), Caspase recruitment domain-containing protein 12 (CARD12), Ice protease-activating factor (IPAF)\u003cbr\u003e\nIsotype: Mouse IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46864377118889,"sku":"659702","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-659702","provider":"Iright","version":"1.0","type":"link"}