{"product_id":"biolegend-661202","title":"Biolegend, 661202, Purified anti-CX3CL1 (Fractalkine) Antibody, 500μg","description":"\u003cp\u003eCX3CL1 is the only member of the CX3C chemokine family that is constitutively expressed in endothelial and epithelial cells, lymphocytes, neurons and microglial osteoblasts. CX3CL1 exists in two forms: as a membrane-anchored form of approximately 100 kD or as a shed 80-95 kD glycoprotein. The chemokine domain is roughly 8 kD, but it migrates at about 13 kD. Its membrane bound form promotes the firm adhesion of rolling leucocytes onto the vessel wall, while its soluble form serves as a potent chemoattractant for CX3CR1-expressing cells such as NK cells, CD14 + monocytes, cytotoxic effector T cells, B cells, neurons, microglia, smooth muscle cells, and tumor cells. The CX3CL1\/CX3CR1 axis plays an important role in the pathogenesis of several inflammatory diseases such as allergic pathologies, rheumatoid arthritis, atherosclerosis and tumorigenesis.\u003cbr\u003e\n500μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Recombinant human CX3CL1\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: WB - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.5 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nRRID: AB_2563890 (BioLegend Cat. No. 661201) AB_2563891 (BioLegend Cat. No. 661202)\u003cbr\u003e\nStructure: Chemokine, membrane bound form includes an extracellular NH2-terminal domain, a mucin-like stalk, a transmembrane α helix, and a short cytoplasmic tail. Full-length CX3CL1 contains over 300 amino acids and is highly glycosylated.\u003cbr\u003e\nDistribution: Cell membrane, secreted.\u003cbr\u003e\nFunction: CX3CL1 has potent chemoattractant activity for T cells, monocytes, and natural killer cells, and the expression of CX3CL1 in activated endothelial cells promotes strong adhesion of those leukocytes. CXC3L1 is induced in endothelial cells by proinflammatory cytokines (TNF-α, IFN-γ, IL-1β) and LPS.\u003cbr\u003e\nInteraction: Monocytic cells, smooth muscle cells, T cells, B cells, NK cells, microglia, and astrocytes.\u003cbr\u003e\nLigand\/Receptor: CX3CR1\u003cbr\u003e\nCell Type: B cells\u003cbr\u003e\nBiology Area: Cell Adhesion, Cell Biology, Immunology, Signal Transduction\u003cbr\u003e\nMolecular Family: Cytokines\/Chemokines\u003cbr\u003e\nAntigen References: 1. Ferretti E, et al. 2014. Mediators Inflamm. 2014:480941. 2. Apostolakis S, et al. 2013. Acta Pharmacol Sin. 34:1251. 3. Julia V, et al. 2012. Allergy. 67:1106. 4. Bazan JF, et al. 1997. Nature 6617:640. 5. Hundhausen C, et al. 2003. Blood 102:1186. 6. Sato E, et al. 2006. Ann. Rheum. Dis. 65:1257. 7. Jones B, et al. 2011. Front. Immunol. 2:82.\u003cbr\u003e\nGene ID: 6376\u003cbr\u003e\nUniProt: View information about CX3CL1 on UniProt.org\u003cbr\u003e\nClone: L393H11\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Chemokine (C-X3-C Motif) Ligand 1 (CX3CL1), ABCD-3, Fractalkine, Neurotactin (NTT, NTN), Small Inducible Cytokine Subfamily D (Cys-X3-Cys), Member-1 (SCYD1), CXC3C\u003cbr\u003e\nIsotype: Mouse IgG2b, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866123030697,"sku":"661202","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-661202","provider":"Iright","version":"1.0","type":"link"}