{"product_id":"biolegend-667701","title":"Biolegend, 667701, Purified anti-BIRC3 (C-IAP2) Antibody, 25μg","description":"\u003cp\u003eBaculoviral IAP Repeat Containing Protein 3, or BIRC3, is a member of the Inhibitor of apoptosis (IAP) family of proteins initially characterized as a component of the TNFα receptor signaling complex. BIRC3 functions as an E3 ubiquitin ligase, polyubiqiutinating target proteins such as RIP kinases, caspases, TRAF1, and BCL10. Loss of BIRC3 results in a significant impairment of TNFα-mediated NF-κB activation signaling cascades.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Human, Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Partial recombinant protein corresponding to amino acids 355-604 of human BIRC\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: WB - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.1 - 1.0 µg\/mL. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: This clone has not been tested for IP. This clone failed to test 4% PFA-fixed Ramos cells permabilized with either methanol or Triton X-100; we do not recommend using the clone for ICC. This clone produced additional bands between ~65 and 95 kD when tested by WB. These bands were also observed with a control antibody raised with a separate epitope. These bands may represent ubiquitinated BIRC3.\u003cbr\u003e\nProduct Citations: Soh KT, et al. 2016. Curr Protoc Cytom. 7.45.1:75. PubMed Banga R, et al. 2023. Cell Host Microbe. 1714:31. PubMed\u003cbr\u003e\nRRID: AB_2814506 (BioLegend Cat. No. 667701) AB_2814507 (BioLegend Cat. No. 667702)\u003cbr\u003e\nStructure: BIRC3 is a 604 amino acid protein with a predicted molecular weight of 68 kD.\u003cbr\u003e\nDistribution: Nucleus, cytoplasm\/widely expressed\u003cbr\u003e\nFunction: Inhibits apoptosis\u003cbr\u003e\nBiology Area: Apoptosis\/Tumor Suppressors\/Cell Death, Cell Biology, Innate Immunity, Signal Transduction, Ubiquitin\/Protein Degradation\u003cbr\u003e\nAntigen References: Seitz CS, et al. 2000. J. Clin. Invest. 105:253. Contze DB, et al. 2005. Mol Cel Biol. 25:3384. Rahal R, et al. 2014. Nat. Med. 20:87.\u003cbr\u003e\nGene ID: 330\u003cbr\u003e\nUniProt: View information about BIRC3 on UniProt.org\u003cbr\u003e\nClone: W17221A\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Baculoviral IAP Repeat Containing 3, Inhibitor Of Apoptosis Protein 1, Apoptosis Inhibitor 2, TNFR2-TRAF-Signaling Complex Protein 1, RNF49, MIHC, API2\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866315673769,"sku":"667701","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-667701","provider":"Iright","version":"1.0","type":"link"}