{"product_id":"biolegend-669902","title":"Biolegend, 669902, Purified anti-Cleaved PARP (Asp214) Recombinant Antibody, 100μg","description":"\u003cp\u003ePARP (ADP-Ribose) Polymerase (PARP) is a 113 kD nuclear protein important for mediating the normal cellular response to DNA damage and repair in response to environmental stress. PARP catalyzes the transfer of ADP-ribose units from NAD⁺ to various nuclear proteins, including topoisomerases, histones, and PARP itself, by poly(ADP-ribosyl)ation. Following DNA damage, this protein is cleaved by ICE-like caspases, such as caspase-3 and -7. In humans, this PARP cleavage occurs between Asp214 and Gly215, yielding an 89 kD (from the carboxy-terminal catalytic domain) and a 24 kD fragment (from the amino-terminal DNA binding domain). PARP is an important regulator of cellular differentiation, proliferation, and tumor transformation, and PARP cleavage is considered a classical characteristic of cells undergoing apoptosis. Additionally, this enzyme may be the site of mutation in Fanconi anemia, and may contribute to the pathophysiology of type I diabetes.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Recombinant\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Proprietary synthetic peptide\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: WB - Quality tested ICC, ICFC - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 1.0 µg per ml. For immunocytochemistry, a concentration of 1.0 μg\/ml is recommended. For intracellular flow cytometry using our True-Phos™ Perm Buffer in Cell Suspensions Protocol, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: The QA17A17 antibody reacts with the 89 kD (cleaved at Asp214) fragment of human PARP1. It does not react with full-length PARP1. Fixation and permeabilization using the True-Nuclear Buffer Set and Intracellular Staining Permeabilization Wash Buffer (10X) have also been confirmed to work in addition to the True-Phos™ Perm Buffer during in-house testing and development to detect cleaved PARP (Asp214).\u003cbr\u003e\nAdditional Product Notes: Clone QA17A17 was tested for ICC using PFA-fixed cells permeabilized with either methanol or Triton X-100. Both methods facilitated strong staining.\u003cbr\u003e\nRRID: AB_2814516 (BioLegend Cat. No. 669901) AB_2814517 (BioLegend Cat. No. 669902)\u003cbr\u003e\nStructure: The large fragment of cleaved PARP is an 801 amino acid product with a predicted molecular weight of 89 kD\u003cbr\u003e\nDistribution: Nuclear\u003cbr\u003e\nFunction: Molecular “nick sensor”; base excision repair; catalyzes poly(ADP-ribosyl)ation of acceptor proteins involved in chromatin architecture; DNA metabolism; protein modification may enhance or repress transcription\u003cbr\u003e\nInteraction: Component of a base excision repair complex containing at least XRCC1, PARP2, POLB and LIG3. Heterodimerizes with PARP2, interacts with PARP3, modified TATA-BP, YY1, Sp1, NF-B, p53 and others\u003cbr\u003e\nBiology Area: Apoptosis\/Tumor Suppressors\/Cell Death, Cell Biology, DNA Repair\/Replication, Transcription Factors\u003cbr\u003e\nMolecular Family: Nuclear Markers\u003cbr\u003e\nAntigen References: Satoh M, Lindahl T. 1992. Nature. 356:356. PubMed Kaufmann S, et al. 1993. Cancer Res. 53:3976. PubMed Lazebnik Y, et al. 1994. Nature. 371:347. PubMed D’Amours D, et al. 1999. Biochem J. 342:249. PubMed Chaitanya G, et al. 2010. Cell Commun Signal. 8:31. PubMed\u003cbr\u003e\nGene ID: 142\u003cbr\u003e\nUniProt: View information about Cleaved PARP on UniProt.org\u003cbr\u003e\nClone: QA17A17\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: PARP1, Poly (ADP-ribose) polymerase, PPOL, ARTD1, ADPRT, EC 2.4.2, ADP-Ribosyltransferase (NAD+;Poly (ADP-Ribose) Polymerase), Poly (ADP-Ribose) Polymerase Family, Member 1, DNA ADP-Ribosyltransferase PARP1, Poly[ADP-Ribose] Synthase 1\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866175754409,"sku":"669902","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-669902","provider":"Iright","version":"1.0","type":"link"}