{"product_id":"biolegend-670002","title":"Biolegend, 670002, Purified anti-Cyclophilin D Antibody, 100μg","description":"\u003cp\u003eCyclophilin D is one of the three components of the permeability transition pore (PTP) localized in the mitochondrial matrix. The opening of the PTP is a key event in the initiation of cell death which leads to mitochondrial swelling, outer membrane rupture, and the release of apoptotic regulators such as cytochrome C. Cyclophilin D promotes mitochondrial permeability transition (mPT) in response to increased Ca 2+ level. Cyclophilin D deficient mice have shown desensitization to mTP and require twice as much Ca 2+ intake for the opening of the PTP compared to wild-type mice.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Human, Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Partial recombinant human PPIF protein (30-207 a.a.)\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: WB - Quality tested ICC - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.25 - 1.0 µg per ml. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg\/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: This clone recognizes human cyclophilin D This clone failed IP testing\u003cbr\u003e\nProduct Citations: Kim S, et al. 2022. Cell Stress Chaperones. 27:573. PubMed\u003cbr\u003e\nRRID: AB_2814520 (BioLegend Cat. No. 670001) AB_2814521 (BioLegend Cat. No. 670002)\u003cbr\u003e\nStructure: Cyclophilin D is a 207 amino acid protein with a predicted molecular weight of 22 kD\u003cbr\u003e\nDistribution: Mitochondrial matrix\/Ubiquitously expressed\u003cbr\u003e\nFunction: Apoptotic regulator\u003cbr\u003e\nBiology Area: Cell Death, Mitochondrial Function\u003cbr\u003e\nMolecular Family: Mitochondrial Markers\u003cbr\u003e\nAntigen References: Baines C, et al. 2005. Nature. 434:658 Schinzel A, et al. 2005. PNAS. 102:12005 Basso E, et al. 2005. J BioL Chem. 280:18558\u003cbr\u003e\nGene ID: 10105\u003cbr\u003e\nUniProt: View information about Cyclophilin D on UniProt.org\u003cbr\u003e\nClone: W17239A\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Peptidylprolyl Isomerase F, Peptidyl-Prolyl Cis-Trans Isomerase F, Mitochondrial, Mitochondrial Cyclophilin, Cyclophilin F, Rotamase F, PPIase F, CyP-M, CYP3, CypD, Cyclophilin 3, Cyp-D, CyP-D, PPIF\u003cbr\u003e\nIsotype: Rat IgG2a, λ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866327863465,"sku":"670002","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-670002","provider":"Iright","version":"1.0","type":"link"}