{"product_id":"biolegend-671202","title":"Biolegend, 671202, Purified anti-PKCι Antibody, 100μg","description":"\u003cp\u003eMembers of the protein kinase c family (PKC) are serine\/threonine protein kinases with a broad set of substrates that can regulate diverse cellular processes. PKCι, also referred to as PKCι\/λ, is a member of the atypical subfamily of PKC, which is characterized by complete independence of calcium and diacylglycerol as second messengers. It has been shown to play a role in cancer survival through multiple mechanisms: a. phosphorylation of the DNA-binding domain of FoxO1 resulting in transcriptional reprogramming of c-myc, which leads to increased proliferation of angiosarcoma cells, b. regulating membrane trafficking of type 1-matrix metalloprotease from late endosomal compartments to the plasma membrane, and c. mediating pro-survival by phosphorylating and inhibiting the pro-apoptotic factor BAD in glioblastomas. PKCι is also required for resistance to anti-apoptotic drugs in BCR-Abl-dependent leukemia and may represent a viable target for combinatorial therapies.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Human, Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Partial recombinant human PKCι protein.\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: WB - Quality tested IP - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 1.0 µg\/mL. For immunoprecipitation, the suggested use of this reagent is 2.5 µg\/test. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: This clone was tested for ICC using HeLa cells fixed with 4% PFA and permeabilized with methanol or Triton X-100; it failed due to weak staining.\u003cbr\u003e\nRRID: AB_2820114 (BioLegend Cat. No. 671201) AB_2820115 (BioLegend Cat. No. 671202)\u003cbr\u003e\nStructure: PKCι is a 596 amino acid protein with a predicted molecular weight of 68 kD.\u003cbr\u003e\nDistribution: Cytosol and Nucleus\/Ubiquitously expressed\u003cbr\u003e\nFunction: Protein Kinase\/Cell Signaling\u003cbr\u003e\nBiology Area: Cell Biology\u003cbr\u003e\nMolecular Family: Protein Kinases\/Phosphatase\u003cbr\u003e\nAntigen References: Nayak RC, et al. 2019. Nat. Commun. 10:46. Riddell M, et al. 2018. Nat. Commun. 9:5357. Rosse C, et al. 2014. Proc Natl Acad Sci U S A. 111:E1872. Desai S, et al. 2011. Biochim Biophys Acta. 1813:1190. Ishiguro H, et al. 2009. Proc Natl Acad Sci U S A. 106:16369.\u003cbr\u003e\nGene ID: 5584\u003cbr\u003e\nUniProt: View information about PKCiota on UniProt.org\u003cbr\u003e\nClone: W18205A\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Protein Kinase C iota, Atypical Protein Kinase C-lambda\/iota, Protein Kinase C iota Type, PRKC-lambda\/iota, aPKC-lambda\/iota, atypical protein kinase C, aPKC, aPKCλ\/ι\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866336415913,"sku":"671202","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-671202","provider":"Iright","version":"1.0","type":"link"}