{"product_id":"biolegend-674010","title":"Biolegend, 674010, Alexa Fluor® 647 anti-Nanog Antibody, 100μg","description":"\u003cp\u003eNanog is a transcription factor that plays key roles in the maintenance of pluripotency and self-renewal in embryonic stem cells. Nanog expression is regulated by other transcription factors, which include Sox2 and Oct-4. It also plays a role in epithelial malignancy and is enriched in cancer stem cells. The ablation of nanog is sufficient to reduce the cancer stem cell pool.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Full length human recombinant protein expressed in bacteria\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.\u003cbr\u003e\nConcentration: 0.5 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: ICFC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Nuclear™ Transcription Factor Staining Protocol. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm \/ 635 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses\u003cbr\u003e\nExcitation Laser: Red Laser (633 nm)\u003cbr\u003e\nApplication Notes: NOTE: For flow cytometric staining with this clone, True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended. This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing).\u003cbr\u003e\nProduct Citations: Chen Z, et al. 2024. J Extracell Vesicles. e12409:13. PubMed Ababneh NA, et al. 2020. Stem Cell Research. 49:102000. PubMed Ababneh NA, et al. 2020. Stem Cell Research. 72.77986111. PubMed Ibuki M, et al. 2019. Regen Ther. 0.551388889. PubMed Ababneh NA, et al. 2020. Stem Cell Research. 48:101967. PubMed\u003cbr\u003e\nRRID: AB_2632605 (BioLegend Cat. No. 674010)\u003cbr\u003e\nStructure: 305 amino acids with a predicted molecular weight of approximately 35 kD.\u003cbr\u003e\nDistribution: Nuclear and cytoplasmic. Expressed by embryonic stem (ES) cells or cancer stem cells. Expression decreases with ES differentiation.\u003cbr\u003e\nFunction: Transcription regulator that plays key roles in the maintenance of pluripotency and self-renewal in embryonic stem cells.\u003cbr\u003e\nInteraction: Interacts with SMAD1, SALL4, and ZNF281\/ZFP281.\u003cbr\u003e\nCell Type: Embryonic Stem Cells\u003cbr\u003e\nBiology Area: Apoptosis\/Tumor Suppressors\/Cell Death, Cell Biology, Cell Cycle\/DNA Replication, Signal Transduction, Stem Cells, Transcription Factors\u003cbr\u003e\nAntigen References: 1. Hwang CC, et al. 2014. PLoS One 9:e105676. 2. Torres-Padilla ME, et al. 2014. Development 141:2173. 3. Iv Santaliz-Ruiz LE, et al. 2014. Int. J. Cancer 12:2741. 4. Wang ML, et al. 2013. Onco. Targets Ther. 6:1207. 5. Kim J, et al. 2008. Cell 132:1049. 6. Takahashi K, et al. 2007. Nat. Protoc. 2:3081. 7. Yu J, et al. 2007. Science 318:1917.\u003cbr\u003e\nGene ID: 79923\u003cbr\u003e\nUniProt: View information about Nanog on UniProt.org\u003cbr\u003e\nClone: 23D2-3C6\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Homeobox Transcription Factor Nanog, Nanog Homeobox, Homeobox Protein NANOG, Homeobox Transcription Factor Nanog-Delta 48\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46860871958697,"sku":"674010","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-674010","provider":"Iright","version":"1.0","type":"link"}