{"product_id":"biolegend-679702","title":"Biolegend, 679702, Purified anti-IGFBP-3 Antibody, 100μg","description":"\u003cp\u003eThe insulin-like growth factor system includes two receptors (IGF-1R and IGF-2R), two ligands (IGF-1 and IGF-2), and seven binding proteins (IGFBP1-7). The binding proteins are produced in the liver and bind to the IGFs with different affinities, thus regulating the interaction of the IGFs with their receptors. Insulin-like growth factor binding protein-3 (IGFBP-3) is the major serum carrier protein for IGF proteins. IGFBP-3 sequesters IGF in a stable ternary complex with the acid-labile subunit (ALS). IGFBP-3 binds IGF-1 with high affinity and specificity and modulates IGF-1 action. IGFBP-3 undergoes limited enzymatic proteolysis to generate small N-terminal fragments. These proteolytic products lose their affinity for IGF-1 and IGF-2. Nevertheless, they preserve IGF independent activity. In that sense, a 16 kD fragment (1-95-IGFBP-3) and the full length molecule can potentiate apoptosis in colorectal epithelial cells, and this apoptotic process is associated with the inhibition of TNF-α-induced NF-κB activity. In addition, IGFBP-3 has been detected in the cell nucleus and interacts with 9-cis retinoic acid receptor-alpha (RXR-α), which is a nuclear receptor that is required for the apoptosis-inducing effects of IGFBP-3. IGFBP-3R physically interacts and activates caspase-8 and mediates IGFBP-3-induced apoptosis and tumor suppression.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Human IGFBP-3, amino acids (Gly28-Glu291) (Accession# X64875) with an N-terminal Met, was expressed in 293E cells.\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: WB - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nRRID: AB_2566243 (BioLegend Cat. No. 679702)\u003cbr\u003e\nStructure: 291 amino acids with a predicted molecular weight of approximately 32 kD. The glycosylated form migrates at approximately 45-50 kD by SDS-PAGE.\u003cbr\u003e\nDistribution: IGFBP-3 is expressed by most of the tissues and is present in plasma.\u003cbr\u003e\nFunction: Transports insulin like growth factors (IGFs) and protects them from rapid clearance and\/or degradation as well as modulating IGFs bioavailability. IGFBP-3 induces apoptosis in vitro and suppresses tumors in vivo. IGFBP-3 is induced by growth hormones, TGF-β, vitamin D, retinoids, and anti-estrogens.\u003cbr\u003e\nInteraction: Interacts with XLKD1, IGF2, IGF1, ALS, HN, and TMEM219.\u003cbr\u003e\nLigand\/Receptor: IGFBP-3R (TMEM219).\u003cbr\u003e\nBiology Area: Apoptosis\/Tumor Suppressors\/Cell Death, Cell Biology, Cell Cycle\/DNA Replication, Signal Transduction\u003cbr\u003e\nAntigen References: 1. Cohen P, et al. 1992. J. Clin. Endocrinol. Metab. 75:1046. 2. Lee KW and Cohen P. 2002. J. Endocrinol. 175:33. 3. Yan X, et al. 2004. J. Biol. Chem. 279:53232. 4. Shahjee H, et al. 2008. Growth Horm. IGF Res. 18:188-97. 5. Berrigan D, et al. 2009. Growth Horm. IGF Res. 2:146. 6. Ingermann AR, et al. 2010. J. Biol. Chem. 285:30233. 7. Forbes BE, et al. 2012. Front. Endocrinol. 3:38. 8. Baxter RC, et al. 2014. Nat. Rev. Cancer 14:329.\u003cbr\u003e\nGene ID: 3486\u003cbr\u003e\nUniProt: View information about IGFBP-3 on UniProt.org\u003cbr\u003e\nClone: M6501G03\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Insulin-like growth factor binding protein 3, IGF-Binding Protein 3, Growth Hormone-Dependent Binding Protein, Binding Protein 29, IBP3, BP-53, IGFBP3\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866124308649,"sku":"679702","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-679702","provider":"Iright","version":"1.0","type":"link"}