{"product_id":"biolegend-680601","title":"Biolegend, 680601, Purified anti-V5-tag Antibody, 25μg","description":"\u003cp\u003eEpitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. The V5 tag is a 14 amino acid peptide derived from a small epitope (Pk) on the P and V proteins of simian virus 5 (SV5), a member of the paramyxovirus family. Because of its small size, the addition of a V5 tag sequence (GKPIPNPLLGLDST) to a given gene creates a stable fusion product that does not appear to interfere with the biochemical properties of the protein. Many recombinant proteins have been engineered to express a short sequence derived from the V5 molecule known as V5-tag.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Epitope tag\u003cbr\u003e\nReported Reactivity: Species independent\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: WB - Quality tested IP - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 0.5 µg per ml. For immunoprecipitation, the suggested use of this reagent is 1-5 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nProduct Citations: Bussey KA, et al. 2018. PLoS Pathog. 11:43. PubMed Hertzog J, et al. 2022. EMBO J. :e109217. PubMed Pham TH, et al. 2020. Cancer Discov. . PubMed Lou Y, et al. 2020. Cell Mol Immunol. 1.439583333. PubMed Stempel M, et al. 2019. EMBO J. 38:. PubMed\u003cbr\u003e\nRRID: AB_2566386 (BioLegend Cat. No. 680601) AB_2566387 (BioLegend Cat. No. 680602)\u003cbr\u003e\nStructure: 14 amino acid peptide (GKPIPNPLLGLDST)\u003cbr\u003e\nFunction: The V5 tag can be used to detect the expression of recombinant proteins in bacteria, yeast, insects, and mammalian cell systems.\u003cbr\u003e\nBiology Area: Cell Biology\u003cbr\u003e\nAntigen References: 1. Uhlen M, et al. 1990. Meth Enzymol. 185:129. 2. Hanke T, et al. 1995. J. Virol. Methods 53:149. 3. Southern JA, et al. 1991. J. Gen. Virol. 72:1551. 4. Thomas SM, et al. 1988. Cell 54:891.\u003cbr\u003e\nGene ID: NA\u003cbr\u003e\nUniProt: View information about V5-tag on UniProt.org\u003cbr\u003e\nClone: 7\/4\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: GKPIPNPLLGLDST epitope tag\u003cbr\u003e\nIsotype: Mouse IgG2b, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862483292329,"sku":"680601","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-680601","provider":"Iright","version":"1.0","type":"link"}