{"product_id":"biolegend-686901","title":"Biolegend, 686901, Purified anti-ERK1\/2 Antibody, 25μg","description":"\u003cp\u003eERK1\/2 are members of the mitogen-activated kinases (MAPKs) that are serine\/threonine protein kinases. ERK1\/2 can be activated by a range of extracellular stimuli, such as mitogen, growth factors, neurotransmitters, chemokines, and cytokines, through receptor tyrosine kinases (RTK), G protein-coupled receptors (GPCRs), or protein kinase C (PKC). Upon stimulation, ERK1\/2 are phosphorylated by the upstream kinase MEK on residues Thr202 and Tyr204 and in turn phosphorylate many other downstream molecules that are involved in a range of cellular processes such as cell proliferation, differentiation, motility and cell death.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Human, Mouse, Drosophila\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Partial human ERK1 recombinant protein (180-379 a.a.) expressed in E. coli.\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: WB - Quality tested KO\/KD-WB, ICC - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 – 2.0 µg\/ml in human, mouse, and Drosophila. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg\/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: Reactivity to Drosophila was only verified with the purified format. The drosophila homolog of mammalian ERK1\/2 is the 43 kD mitogen-activated protein kinase ERK-A.\u003cbr\u003e\nProduct Citations: Chyuan IT, et al. 2024. J Biomed Sci. 33:31. PubMed Zhang A, et al. 2022. EMBO J. 41:e109187. PubMed McFall T, et al. 2021. Cell Rep. 37:110096. PubMed McFall T, et al. 2022. NPJ Precis Oncol. 6:86. PubMed Zhang Q, et al. 2021. Transl Res. 235:129. PubMed Bent EH, et al. 2021. Nat Commun. 12:6218. PubMed Lucas ED, et al. 2020. Cell Reports. 33(2):108258. PubMed\u003cbr\u003e\nRRID: AB_2861071 (BioLegend Cat. No. 686901) AB_2629535 (BioLegend Cat. No. 686902)\u003cbr\u003e\nStructure: ERK1 and ERK2 encode 42 and 44 kD protein kinases and share 85% homology.\u003cbr\u003e\nDistribution: Nucleus and cytoplasm.\u003cbr\u003e\nFunction: ERK1\/2 are broadly expressed protein kinases that are involved in proliferation, differentiation, motility, and cell death. In response to extracellular stimuli, ERK1\/2 are phosphorylated at Thr202 and Tyr204 and in turn transduce signals to downstream substrates.\u003cbr\u003e\nInteraction: Interacts with ADAM15, ARRB2, CANX, DAPK1, HSF4, IER3, MAP2K1\/MEK1, MORG1, NISCH, SGK1, TPR, CDKN2AIP, PEA15, and MKNK2.\u003cbr\u003e\nBiology Area: Apoptosis\/Tumor Suppressors\/Cell Death, Cell Biology, Signal Transduction\u003cbr\u003e\nMolecular Family: Protein Kinases\/Phosphatase\u003cbr\u003e\nAntigen References: 1. Futran AS, et al. 2013. Curr. Biol. 23:R972. 2. Mendoza MC, et al. 2011. Trends Biochem. Sci. 36:320. 3. Chambard JC, et al. 2007. Biochim. Biophys. Acta. 1773:1299. 4. Roux PP, et al. 2004. Microbiol. Mol. Biol. Rev. 68:320. 5. Torii S, et al. 2004. J. Biochem. 136:557. 6. Clark EA, et al. 1996. J. Biol. Chem. 271:14814.\u003cbr\u003e\nGene ID: 55955594\u003cbr\u003e\nUniProt: View information about ERK1\/2 on UniProt.org\u003cbr\u003e\nClone: W15133B\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Extracellular signal-regulated kinase 1\/2, Mitogen-activated protein kinase MAPK3\/MAPK1, p44ERK1\/p42ERK2, p44-MAPK\/p42MAPK, PRKM3\/PRKM2, ERK\u003cbr\u003e\nIsotype: Rat IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866299125929,"sku":"686901","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-686901","provider":"Iright","version":"1.0","type":"link"}