{"product_id":"biolegend-687802","title":"Biolegend, 687802, Purified anti-14-3-3 ζ\/δ Antibody, 100μg","description":"\u003cp\u003eIn mammals, proteins of the 14-3-3 family include isoforms β, γ, ε, σ, ζ, τ, and η. 14-3-3 δ isoform is the phosphorylated form of 14-3-3 ζ. These highly conserved proteins form homo- or heterodimers with many transcription factors, metabolic enzymes, kinases, phosphatases, and other signaling molecules, generally through a phospho-Ser\/Thr motif. The binding regulates the location, stability, interaction with other molecules, and phosphorylation status of the target proteins which suggests the important roles for these 14-3-3 family proteins on regulation of cellular functions and responses. Growing data demonstrate that the specific temporal and spatial distributions of 14-3-3 family proteins are critical for developmental processes; and in some cancers, the regulation of these proteins are different from healthy controls. 14-3-3 ζ interacts with IRS1 protein and is involved in the PI3K-Akt signaling pathway.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Human, Mouse, Rat, Drosophila\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Full length human 14-3-3 ζ\/δ recombinant protein expressed in E. coli.\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: WB - Quality testedICC - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 – 2.0 µg\/ml in human, mouse, and rat and 0.25 – 2.0 µg\/ml in Drosophila. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg\/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: 14-3-3 family members share a high homology in amino acid sequences. Hence, this clone may recognize other 14-3-3 family members with predicted MW of 27, 28kD.Reactivity to Drosophila was only verified with the purified format.\u003cbr\u003e\nRRID: AB_2617006 (BioLegend Cat. No. 687802)\u003cbr\u003e\nStructure: 245 amino acids with a predicted molecular weight of 27.7 kD.\u003cbr\u003e\nDistribution: Cytoplasm.\u003cbr\u003e\nFunction: 14-3-3 ζ\/δ is an adaptor protein, binding to a large number of Ser\/Thr phosphorylated proteins. It participates in several signaling pathways and regulates the activity of the binding partners.\u003cbr\u003e\nInteraction: Forms a homooligomer or a heterodimer with other isoforms. Interacts with CDK16, BSPRY, WEE1, SAMSN1, MLF1, ITGB2, BCL2L11, FOXO4, NOXA1, SSH1, ARHGEF2, BAX, RAF1, TP53, ABL1, AANAT, AKT1, GAB2, TLK2, and DAPK2.\u003cbr\u003e\nBiology Area: Cell Biology, Signal Transduction\u003cbr\u003e\nAntigen References: 1. Funami K, et al. 2016. Mol. Immunol. 73:60. 2. Bonet R, et al. 2013. J. Mol. Biol. 425:3060. 3. Hong HY, et al. 2010. Mol. Cells 29:305. 4. Danes CG, et al. 2008. Cancer Res. 68:1760. 5. Niemantsverdriet M, et al. 2008. Oncogene. 27:1315. 6. Bialkowska K, et al. 2003. J. Biol. Chem. 278:33342.\u003cbr\u003e\nGene ID: 7534\u003cbr\u003e\nUniProt: View information about 14-3-3 zeta\/delta on UniProt.org\u003cbr\u003e\nClone: W15121A\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Protein kinase C inhibitor protein 1, KCIP-1, HEL4, YWHAD, HEL-S-3, HEL-S-93, 14-3-3-zeta\u003cbr\u003e\nIsotype: Rat IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866298994857,"sku":"687802","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-687802","provider":"Iright","version":"1.0","type":"link"}