{"product_id":"biolegend-690502","title":"Biolegend, 690502, Purified anti-SIRT1 Antibody, 100μg","description":"\u003cp\u003eSIRT1, the human homolog of the S. cerevisiae Sir2 protein, functions as an NAD-dependent deacetylase of a number of nonhistone substrates including p53. In response to DNA damage, SIRT1 binds and deacetylates the p53 protein at c-terminal Lys382 residue and attenuates p53-mediated functions. When overexpressed in mouse embryo fibroblasts, SIRT1 antagonizes PML-induced acetylation of p53 and rescues PML-mediated premature cellular senescence. In mammalian cells, SIRT1 appears to control the cellular response to stress by regulating the FOXO family of forkhead transcription factors.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Human, Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Partial human SIRT1 recombinant protein (548-747 a.a.) expressed in E. coli.\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: WB - Quality tested KO\/KD-WB - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nRRID: AB_2629822 (BioLegend Cat. No. 690502)\u003cbr\u003e\nStructure: 747 amino acids with a predicted molecular weight of 81.6 kD and observed of approximately 120 kD. Contains a sirtuin-type deacetylase domain.\u003cbr\u003e\nDistribution: Nucleus, cytoplasm, and PML body.\u003cbr\u003e\nFunction: SIRT1 is a NAD-dependent deacetylase involving in the regulation of cellular metabolism and stress responses.\u003cbr\u003e\nInteraction: Interacts with p53, E1A binding protein p300, HEY2, NF-kB3, Transcription factor HES-1, Forkhead box protein O3A, and PML.\u003cbr\u003e\nBiology Area: Cell Biology, Signal Transduction\u003cbr\u003e\nAntigen References: 1. Li L, Bhatia R. 2015. Curr. Opin. Hematol. 22:324. 2. Qiu G, et al. 2015. FEBS Lett. 589:2034. 3. Chen X, et al. 2015. Immunology 145:455. 4. Simmons GE Jr, et al. 2015. Int. J. Mol. Sci. 16:950. 5. Kauppinen A, et al. 2013. Cell Signal. 25:1939. 6. Paraíso AF, et al. 2013. Mol. Neurobiol. 48:681.\u003cbr\u003e\nGene ID: 23411\u003cbr\u003e\nUniProt: View information about SIRT1 on UniProt.org\u003cbr\u003e\nClone: W16003A\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: NAD-dependent deacetylase sirtuin-1, SIR2-like protein 1 (SIR2L1), Regulatory protein SIR2 homolog 1, hSIRT1, hSIR2\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866300862633,"sku":"690502","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-690502","provider":"Iright","version":"1.0","type":"link"}