{"product_id":"biolegend-693156","title":"Biolegend, 693156, Alexa Fluor® 647 anti-BAK1 Antibody, 100μg","description":"\u003cp\u003eBAK1 (Bcl-2 homologous antagonist\/killer) is a crucial protein involved in regulating apoptosis, or programmed cell death, which is essential for maintaining cellular homeostasis. It functions as a pro-apoptotic factor, promoting cell death in response to various stress signals. BAK1 belongs to the BCL-2 family of proteins and plays a crucial role in the mitochondrial apoptotic process by promoting mitochondrial outer membrane permeabilization, which leads to the release of apoptogenic factors into the cytosol. BAK1 is regulated through interactions with other proteins, such as BCL-2 family members, which can either inhibit or promote its apoptotic activity. Dysregulation of BAK1 has been implicated in several diseases, thus, overexpression of BAK1 can contribute to neurodegenerative and autoimmune diseases by promoting excessive cell death. Conversely, inhibition of BAK1 is associated with the development of cancers, as it allows cells to evade apoptosis and continue proliferating. This product was carefully developed for performance and specificity using the following products from Horizon Discovery, a Revvity company: HAP1 WT Cell Line (Horizon Cat No. Cat. No. C631) and HAP1 BAK1 Knockout cell line (Horizon Cat. No. HZGHC005962c001)\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Recombinant Human BAK1 protein\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.\u003cbr\u003e\nConcentration: 0.5 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: IHC-P - Quality tested ICFC - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 2.5 - 10.0 µg\/mL is suggested. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm \/ 635 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses\u003cbr\u003e\nExcitation Laser: Red Laser (633 nm)\u003cbr\u003e\nAdditional Product Notes: For use of this antibody in intracellular flow cytometry (ICFC), we recommend fixation and permeabilization using True-Phos™ Perm Buffer (Cat. No. 425401). For use of this antibody in immunohistochemistry on formalin-fixed paraffin-embedded tissues (IHC-P), we recommend antigen retrieval using either Citrate Buffer (Cat. No. 420902), or Tris-EDTA pH 9.0 Antigen Retrieval Buffer (Cat. No. 422704).\u003cbr\u003e\nRRID: AB_3720605 (BioLegend Cat. No. 693155)\u003cbr\u003e\nStructure: BAK1 protein consists of 211 amino acids with a predicted molecular weight of 23 kDa.\u003cbr\u003e\nDistribution: Mitochondrial outer membrane\u003cbr\u003e\nFunction: • Pro-apoptotic role: BAK1 promotes apoptosis by facilitating mitochondrial outer membrane permeabilization. • Interaction with BCL-2 family: It interacts with other BCL-2 family proteins to regulate apoptosis. • Release of apoptogenic factors: Upon activation, BAK1 helps release factors from the mitochondria that trigger the apoptotic cascade.\u003cbr\u003e\nInteraction: • BCL-2 and BCL-XL: These anti-apoptotic proteins bind to BAK1, inhibiting its pro-apoptotic activity. • BAX: BAK1 forms heterooligomers with BAX, another pro-apoptotic protein, to promote mitochondrial outer membrane permeabilization. • p53: The tumor suppressor protein p53 interacts with BAK1 in response to cellular stress, enhancing its apoptotic function. • MCL1: This anti-apoptotic protein also interacts with BAK1, regulating its activity. • VDAC2: BAK1 interacts with the mitochondrial voltage-dependent anion channel 2, which is involved in the release of apoptogenic factors\u003cbr\u003e\nBiology Area: Apoptosis\/Tumor Suppressors\/Cell Death, Cancer Biomarkers, Cell Biology, Mitochondrial Function, Neurodegeneration\u003cbr\u003e\nMolecular Family: Mitochondrial Markers\u003cbr\u003e\nAntigen References: Dadsena S, et al. 2021. Biochem Biophys Acta Biomembr. 1863:183716 Shalaby R, et al. 2020. Biomolecules. 10:1638 Ramesh P, et al. 2020. Apoptosis. 25:305 Pena-Blanco A, et al. 2018. FEBS J. 285:416\u003cbr\u003e\nRegulation: • Protein interactions: BAK1 is regulated through its interactions with pro- and anti-apoptotic BCL-2 family members. • Activation by stress signals: It remains inactive until activated by various cellular stress signals. • Post-translational modifications: Phosphorylation and other modifications can influence BAK1's activity and stability.\u003cbr\u003e\nGene ID: 578\u003cbr\u003e\nUniProt: View information about BAK1 on UniProt.org\u003cbr\u003e\nClone: W23097D\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: BCL2 antagonist\/killer 1, BCL2L7, BAK, CDN1, Apoptosis regulator BAK, Bcl-2 homologous antagonist\/killer\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866438127785,"sku":"693156","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-693156","provider":"Iright","version":"1.0","type":"link"}