{"product_id":"biolegend-695302","title":"Biolegend, 695302, Purified anti-EOS Antibody, 100μg","description":"\u003cp\u003eEOS, also known as IKZF4 (Ikaros family zinc finger 4), is a member of the Ikaros family of zinc finger transcription factors, which are key transcriptional repressors during development of lymphocytes. Each of the five Ikaros family members (Ikaros, Helios, Aiolos, Eos and Pegasus) has N-terminal and C-terminal zinc-finger motifs responsible for DNA binding and dimerization, respectively. EOS is a highly conserved DNA-binding protein that recognizes the typical 5'WGGAAT-3' Ikaros-binding sequence. EOS has been implicated in the development of lymphoid cells and nervous systems, as well as mediating FOXP3-dependent gene silencing in CD4 + regulatory T cells though interacting with FOXP3 and CTBP1.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Partial human EOS recombinant protein (271-520 a.a.) expressed in E. coli.\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: WB - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1:1000 - 1:250 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: Clone W15169A does not cross-react with mouse (in-house tested). This antibody recognizes both isoforms of EOS and it also detects a non-specific protein of approximately 75 kD in the K562 cell line tested.\u003cbr\u003e\nRRID: AB_2715762 (BioLegend Cat. No. 695302)\u003cbr\u003e\nStructure: EOS has two isoforms. Isoform 1 has 585 amino acids with a predicted molecular weight of 64.1 kD. Isoform 2 has 544 amino acids with a predicted molecular weight of 59.7 kD. Both isoforms contain six zinc finger domains responsible for DNA-binding or prot\u003cbr\u003e\nDistribution: Nucleus\u003cbr\u003e\nFunction: EOS is a zinc finger transcription factor that is implicated in the development of lymphoid cells and nervous systems.\u003cbr\u003e\nInteraction: EOS forms homodimers or heterodimers with other Ikaros family members. Interacts with CTBP2, SPI1, MITF, FOXP3, and CTBP1.\u003cbr\u003e\nAntigen References: 1. Rieder SA, et al. 2015. J. Immunol. 195:553. 2. Raffin C, et al. 2013. J. Immunol. 191:4619. 3. Paradzik M, et al. 2012. Int. J. Immunopathol. Pharmacol. 25:893. 4. Bettini ML, et al. 2012. Immunity. 36:717. 5. John LB, Ward AC. 2011. Mol. Immunol. 48:1272. 6. Pan F, et al. 2009. Science. 325:1142.\u003cbr\u003e\nGene ID: 64375\u003cbr\u003e\nUniProt: View information about EOS on UniProt.org\u003cbr\u003e\nClone: W15169A\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Ikaros family zinc finger protein 4 (IKZF4), KIAA1782, ZNFN1A4\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866300174505,"sku":"695302","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-695302","provider":"Iright","version":"1.0","type":"link"}