{"product_id":"biolegend-699955","title":"Biolegend, 699955, PhosphoPair STAT1 (Ser727) Antibody Set, 1kit","description":"\u003cp\u003eSTAT1, also known as signal transduction and activator of transcription 1, is a ubiquitously expressed cytoplasmic protein and is activated in response to cytokine signaling, including IFN-α, IFN-γ, EGF, PDGF, and IL-6. Upon activation, STAT1 is phosphorylated by receptor-associated kinases, translocates to the nucleus, and functions as a transcription factor. Phosphorylation at serine 727 is required for full transcriptional activator function. Two isoforms of STAT1, with apparent molecular weights of 88 (STAT1α) and 91 (STAT1β) kD, exist as a result of alternative RNA processing. STAT1 is involved in IFN-mediated immune responses, and STAT1-deficient mice are highly sensitive to bacterial and viral infections.\u003cbr\u003e\n1kit\u003cbr\u003e\nVerified Reactivity: Human, Mouse\u003cbr\u003e\nImmunogen: Please refer to individual product datasheets for immunogen information\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: WB - Quality testeed\u003cbr\u003e\nRecommended Usage: Each lot of antibodies in this set is quality control tested by Western blotting. For Western blotting, the suggested uses of these reagents are as follows: Clone 10C4B40: 1.0 µg\/mL Clone A15158B: 0.5 - 2.5 µg\/mL It is recommended that the reagent be titrated for optimal performance for each application. For recommended usage information for additional applications, please refer to the datasheet of the individual products.\u003cbr\u003e\nApplication Notes: The serine 727 phosphorylation site is only found in STAT1α; it is absent in STAT1β. Therefore, clone A15158B only detects a single band corresponding to the serine 727-phosphorylated α isoform, while clone 10C4B40 detects both α and β isoforms.\u003cbr\u003e\nStructure: Two isoforms of STAT1 exist as a result of alternative splicing. Isoform α is a 750 amino acid protein with a predicted molecular weight of 87 kD; the β isoform is a 712 amino acid protein with a predicted molecular weight of 83 kD.\u003cbr\u003e\nDistribution: Ubiquitously expressed\/Nucleus and cytosol\u003cbr\u003e\nFunction: Phosphorylated in response to cytokine signaling by receptor-associated kinases; translocates to the nucleus to act as a transcription factor. Mediates responses to type I (IFN-α\/β) and II interferon (IFN-γ), EGF, PDGF, and IL-6.\u003cbr\u003e\nInteraction: Forms a homodimer or heterodimers with other family members. Interacts with FAK, MCM3, MCM5, TRADD, BRCA1, KIT, IL-27R, IL-2Rβ, IL-2Rγ, IFNαβR, and c-Src.\u003cbr\u003e\nCell Type: Mesenchymal cells\u003cbr\u003e\nBiology Area: Cell Biology, Immunology, Innate Immunity, Signal Transduction, Stem Cells, Transcription Factors\u003cbr\u003e\nMolecular Family: Nuclear Markers, Phospho-Proteins\u003cbr\u003e\nAntigen References: Durbin JE, et al. 1996. Cell. 84:443-50. Darnell JE Jr, et al. 1994. Science. 264:1415-21. Chen X, et al. 1998. Cell. 93:827-39. Ramana CV, et al. 2000. Oncogene. 19:2619-27.\u003cbr\u003e\nGene ID: 6772\u003cbr\u003e\nUniProt: View information about STAT1 on UniProt.org\u003cbr\u003e\nClone: 10C4B40; A15158B;\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Signal transducer and activator of transcription 1 (STAT1), Transcription factor ISGF-3 components p91\/p84\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46864323641513,"sku":"699955","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-699955","provider":"Iright","version":"1.0","type":"link"}