{"product_id":"biolegend-699956","title":"Biolegend, 699956, PhosphoPair PLK-1 (Thr210) Antibody Set, 1kit","description":"\u003cp\u003ePLK-1 (polo-like kinase 1) is a member of the Polo-like kinase family of serine\/threonine protein kinases. While PLK-1 phosphorylates a broad range of targets, it has been shown to be a critical regulator of cell cycle pathways, ranging from mitotic entry to initiation of cytokinesis. Elevated PLK1 expression is described in many cancers and is associated with poor clinical outcomes. Increased PLK-1 activity is associated with resistance to doxorubicin and gemcitabine in certain cancers. Activation of PLK-1 requires phosphorylation of Thr210 within the T-loop of the catalytic domain by Aurora kinases.\u003cbr\u003e\n1kit\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nImmunogen: Please refer to individual product datasheets for immunogen information\u003cbr\u003e\nFormulation: Phosphate-buffered solutions, pH 7.2, containing 0.09% sodium azide\u003cbr\u003e\nPreparation: The antibodies were purified by affinity chromatography.\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solutions should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: WB - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of antibodies in this set is quality control tested by Western blotting. For Western blotting, the suggested uses of these reagents are as follows: Clone 3F8: 0.2 - 1.0 µg\/mL Clone 2A3: 0.5 - 1.0 µg\/mL It is recommended that the reagent be titrated for optimal performance for each application. For recommended usage information for additional applications, please refer to the datasheet of the individual products.\u003cbr\u003e\nProduct Citations: Chilamakuri R, et al. 2022. Pharmaceuticals (Basel). 15:. PubMed\u003cbr\u003e\nStructure: PLK1 is a 603 amino acid protein with a predicted molecular weight of ~68 kD.\u003cbr\u003e\nDistribution: Ubiquitously expressed; highly expressed in placenta and colon\/Centrosomes and kinetochore\u003cbr\u003e\nFunction: Mitotic signaling\u003cbr\u003e\nAntigen References: 1. Hamanaka R, et al. 1994. Cell Growth Differ. 5:249. 2. Lake RJ, et al. 1993. Mol. Cell. Biol. 13:7793. 3. Holtrich U, et al. 1994. P. Natl. Acad. Sci. USA 91:1736.\u003cbr\u003e\nGene ID: 5347\u003cbr\u003e\nUniProt: View information about PLK-1 on UniProt.org\u003cbr\u003e\nClone: 3F8; 2A3;\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Polo Like Kinase 1,STPK13, Cell Cycle Regulated Protein Kinase\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862396063913,"sku":"699956","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-699956","provider":"Iright","version":"1.0","type":"link"}