{"product_id":"biolegend-799506","title":"Biolegend, 799506, Biotinylated Recombinant Human PD-1-Fc (CD279) Chimera (carrier-free), 100μg","description":"\u003cp\u003eProgrammed death-1 (PD-1) is a type I transmembrane protein initially isolated from apoptosis-induced cells by subtractive hybridization. The amino acid sequence of mouse PD-1 shares 59.4% identity to the human counterpart, and a putative tyrosine kinase-association motif is well-conserved. Its extracellular region consists of a single Ig-like variable (IgV) domain. PD-1 is an inhibitory molecule expressed by activated B and T cells and has been implicated in immune tolerance. PD-L1 and PD-L2 are the ligands for PD-1 and their binding leads to the inhibition of T cell receptor-mediated lymphocyte proliferation and cytokine secretion. PD-1\/PD-L1 interaction suppresses immune responses against autoantigens and tumors, and plays an important role in the maintenance of peripheral immune tolerance. Interaction between PD-1 and PD-L1 promote tolerance by blocking TCR-mediated signaling.  PD-L1 expression by tumor cells allow tumor progression by suppressing antitumor T-cell responses. Antibodies against PD-1 or PD-L1 lead to increased antitumor immunity.\u003cbr\u003e\n100μg\u003cbr\u003e\nSource: Biotinylated Human PD-1, amino acid Leu25-Gln167 (Accession # Q15116.3), with a linker (GS) and a C-terminal human IgG (Pro100-Lys330), followed by a Avi tag, was expressed in 293E cells. Human PD-1-Avi tag was site-specifically biotinylated by enzyme BirA.\u003cbr\u003e\nMolecular Mass: The 391 amino acid recombinant protein has a predicted molecular mass of approximately 43.85 kD. The DTT-reduced and non-reduced protein migrates at approximately 62-70 kD and 120-130 KD by SDS-PAGE, respectively. The predicted N-terminal amino acid is Leu.\u003cbr\u003e\nPurity: \u0026gt; 95%, as determined by Coomassie stained SDS-PAGE.\u003cbr\u003e\nFormulation: 0.22 µm filtered protein solution is in PBS, 5% glycerol.\u003cbr\u003e\nEndotoxin Level: Less than 0.1 EU per µg cytokine as determined by the LAL method\u003cbr\u003e\nConcentration: 25 µg size is bottled at 200 µg\/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.\u003cbr\u003e\nStorage \u0026amp; Handling: Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg\/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze\/thaw cycles.\u003cbr\u003e\nActivity: Biotinylated Human PD-1-Fc binds to immobilized human PD-L2 at 1 µg\/mL (100 µL\/well), with an EC50 of 3 -12 ng\/mL. HRP Avidin (Cat. No. 405103) was used to detect the binding.\u003cbr\u003e\nApplication: Bioassay\u003cbr\u003e\nApplication Notes: BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at [email protected].\u003cbr\u003e\nStructure: Dimer\u003cbr\u003e\nDistribution: Antigen-stimulated T and B cells, regulatory T cells, follicular T and B cells, dendritic cells, and monocytes\u003cbr\u003e\nFunction: Inhibitory receptor on antigen-activated T cells that plays a critical role in induction and maintenance of immune tolerance\u003cbr\u003e\nInteraction: APC, monocytes, dendritic cells, stromal cell, cancer cells\u003cbr\u003e\nLigand\/Receptor: B7-H1 (PD-L1, CD274) and B7-DC (PD-L2, CD273)\u003cbr\u003e\nBioactivity: Measured by its ability to bind to human PD-L2-Fc\u003cbr\u003e\nCell Type: Lymphocytes, T cells\u003cbr\u003e\nBiology Area: Adaptive Immunity, Cancer Biomarkers, Cell Biology, Immuno-Oncology, Immunology, Inhibitory Molecules\u003cbr\u003e\nMolecular Family: CD Molecules, Immune Checkpoint Receptors\u003cbr\u003e\nAntigen References: Ishida Y, et al. 1992. EMBO J. 11:3887-95. Fife BT, et al. 2009. Nature Immunol. 10:1185-92. Freeman GJ, et al. 2000. J Exp Med. 192:1027-34. Latchman Y, et al. 2001. Nat Immunol. 2:261-8. Iwai Y, et al. 2002. Proc Natl Acad Sci USA. 99:12293-7. Dai S, et al. 2014. Cell Immunol. 290:72-9. Tan S, et al. 2017. Nature Commun. 8:14369.\u003cbr\u003e\nGene ID: 5133\u003cbr\u003e\nUniProt: View information about PD-1 on UniProt.org\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Programmed Cell Death Protein 1, PD1, PDCD1, CD279\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46864213442729,"sku":"799506","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-799506","provider":"Iright","version":"1.0","type":"link"}