{"product_id":"biolegend-803017","title":"Biolegend, 803017, Anti-β-Amyloid, 1-16 Antibody (Previously Covance catalog# SIG-39300), 1ml","description":"\u003cp\u003eAlzheimer's disease is characterized by the accumulation of aggregated Aβ peptides in senile plaques and vascular deposits. Aβ peptides are derived from amyloid precursor proteins (APP) through sequential proteolytic cleavage of APP by β-secretases and γ-secretases generating diverse Aβ species. Aβ can aggregate to form soluble oligomeric species and insoluble fibrillar or amorphous assemblies. Some forms of the aggregated peptides are toxic to neurons.\u003cbr\u003e\n1ml\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nPreparation: Ascites\u003cbr\u003e\nConcentration: The concentration is not quantified as this product is sold as undiluted crude mouse ascites fluid. The concentration might vary from lot-to-lot and an estimated concentration would be 1-3 mg\/ml.\u003cbr\u003e\nStorage \u0026amp; Handling: Store at -20°C or below. Upon initial thawing, apportion into working aliquots and store at -20°C or below. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody. Do not store in frost-free freezers.\u003cbr\u003e\nApplication: WB - Quality tested Direct ELISA, IHC-P - Verified IHC-F, EM - Reported in the literature, not verified in house\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested dilution of this reagent is 1:1,000 to 1:20,000. For Direct ELISA, the suggested dilution of this reagent is 1:10,000 to 1:100,000. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, the suggested dilution of this reagent is 1:1,000 to 1:20,000. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: This antibody is reactive to amino acid residue 1-16 of beta amyloid. The epitope lies within amino acids 3-8 of beta amyloid (EFRHDS). This antibody clone has been reported for use in immunohistochemistry of free-floating sections2,13.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Thakker DR, et al. 2009. Proc. Natl. Acad. Sci. USA. 106(11):4501-6. (IHC) PubMed Oddo S, et al. 2005. Proc. Natl. Acad. Sci. USA. 102(8):3046-51. (IHC-other) PubMed Herzig M, et al. 2004. Nat. Neuro. 7(9):954-959. (WB) PubMed Zheng Y, et al. 2012. PLoS One 6:39035. (IHC-F) PubMed Abramowksi D, et al. J Neurosci. 32:1273. (WB) PubMed Forny-Germano L, et al. 2014. J. Neurosci. 34:13629. (WB, IHC) PubMed Gowert NS, et al. 2014. PLoS One 2:e90523. (ICC, EM) PubMed Sandoval-Hernández A, et al. 2015. PLoS One. 10: 0145467. (IHC-F) Kumar R, et al. 2016. Brain. 139:174-92 (WB) Miyamoto T, et al. 2016. J. Biol. Chem. 291:1719-34. (WB) Saito S, et al. 2017. Acta Neuropathol. Commun. 5:26-9. (IHC-P) PubMed Omata Y, et al. 2016. Aging (Albany NY) 8(3):427. (IHC-P) PubMed Peng W, et al. 2016. Neurobiol. Dis. 93:215. (IHC-other) PubMed Mandler M, et al. 2015. PLoS One. e0115237. (WB, IHC, ELISA) PubMed\u003cbr\u003e\nProduct Citations: Fu W, et al. 2018. Exp Ther Med. 128:2252. PubMed Lana E, et al. 2019. Front Mol Neurosci. 12:176. PubMed Ren Z, et al. 2020. Mol Med Rep. 22:201. PubMed Tanaka H, et al. 2020. Nat Commun. 11:507. PubMed Bishay J, et al. 2022. Sci Rep. 12:15287. PubMed Li S, et al. 2022. Neural Plast. 2022:3995227. PubMed Jin J, et al. 2023. Gut Microbes. 15:2167172. PubMed Yao SY, et al. 2023. Br J Pharmacol. 180:1878. PubMed Na D, et al. 2023. Front Neurosci. 17:1106570. PubMed Ren X, et al. 2022. J Neuroinflammation. 19:61. PubMed Tang T, et al. 2022. J Virol. 96:e0168821. PubMed Huang S, et al. 2022. FEBS Lett. 596:1059. PubMed Januário YC, et al. 2022. J Biol Chem. 298:102172. PubMed Cai C, et al. 2022. J Neurosci. 42:6090. PubMed Lucero EM, et al. 2022. iScience. 25:105288. PubMed Hu Y, et al. 2022. Nat Metab. 4:1756. PubMed Reddy TT, et al. 2023. Molecules. 28: . PubMed Bathini P, et al. 2023. Brain Behav Immun Health. 28:100568. PubMed Lin W, et al. 2023. J Neuroinflammation. 20:21. PubMed Chen SH, et al. 2022. Neurosci Bull. 38:290. PubMed Arber C, et al. 2019. Brain Commun. 1:fcz024. PubMed Lai RH, et al. 2021. Aging Cell. 20:e13509. PubMed Forny-Germano L, et al. 2014. J Neurosci. 34:13629-13643. PubMed Beraldo F, et al. 2016. J Biol Chem. 291: 21945 - 21955. PubMed NULL, et al. 2021. Mol Imaging Biol. 23:991. PubMed Cone AS, et al. 2021. Theranostics. 11:8129. PubMed Zhang H, et al. 2016. J Neurosci. 36: 11837 - 11850. PubMed Robert J et al. 2017. eLife. 6 pii: e29595. PubMed Mukherjee J, et al. 2021. Synapse. 75:e22183. PubMed Zheng Y, et al. 2012. PLoS One. 7:e39035. PubMed Soto-Mercado V, et al. 2020. J Alzheimers Dis. . PubMed Shen Y, et al. 2020. Theranostics. 10:11794. PubMed Na H, et al. 2021. Am J Alzheimers Dis Other Demen. 36:15333175211012867. PubMed Shen Q, et al. 2022. Aging Cell. 21:e13573. PubMed Fujita K, et al. 2016. Sci Rep. 6:31895. PubMed Gowert N, et al. 2014. PLoS One. 9:e90523. PubMed Suh J, et al. 2020. Cell. 178(5):1159-1175.e17.. PubMed Nguyen GAH, et al. 2022. Molecules. 27:. PubMed Shang Y, et al. 2022. Acta Neuropathol. 144:911. PubMed Lyra E Silva NM, et al. 2021. Transl Psychiatry. 251:11. PubMed Benthem SD, et al. 2020. Current Biology. 30(13):2588-2601.e5. PubMed Cheng WH, et al. 2019. Alzheimers Res Ther. 11:6. PubMed Soto-Mercado V, et al. 2021. Biomolecules. 11:. PubMed Abramowski D, et al. 2012. J Neurosci. 32:1273-1283. PubMed Zhang J, et al. 2021. Aging Cell. 20:e13380. PubMed Moelgg K, et al. 2021. Biomolecules. 11:. PubMed Zhang X, et al. 2020. Sci Adv. 6:00. PubMed Kaur H, et al. 2021. Bioorg Med Chem Lett. 46:128164. PubMed Casali BT, et al. 2020. Neurobiol Dis. 142:104956. PubMed Quartey MO, et al. 2021. Sci Rep. 0.757638889. PubMed Klohs J, et al. 2016. J Cereb Blood Flow Metab. 36: 1614 - 1624. PubMed Qiu Y, et al. 2022. Front Aging Neurosci. 14:896522. PubMed Kumar S, et al. 2022. NPJ Genom Med. 7:47. PubMed Chan E, et al. 2016. Sci Rep. 6: 26119. PubMed Lee CYD et al. 2018. Neuron. 97(5):1032-1048 . PubMed Ippati S, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed Boeddrich A et al. 2018. Cell chemical biology. 26(1):109-120 . PubMed Zhang Y, et al. 2022. Bioact Mater. 11:192. PubMed Seino S, et al. 2018. Biomed Res. 39:105. PubMed Lin H, et al. 2022. Front Aging Neurosci. 14:885145. PubMed Scholtzova H, et al. 2017. J Neurosci. 37(4):936-959. PubMed Heiss J, et al. 2016. Cereb Cortex. 10.1093\/cercor\/bhw188. PubMed Meilandt WJ, et al. 2020. J Neurosci. 40:1956. PubMed Casali BT, et al. 2018. J Neuroinflammation. 15:43. PubMed Arber C, et al. 2021. Cell Reports. 34(2):108615. PubMed Jang J, et al. 2017. Biochem Biophys Res Commun. 10.1016\/j.bbrc.2017.08.127. PubMed Xiao Zhang et al. 2017. The Journal of Neuroscience. 37(19):4928-4941 . PubMed Actor-Engel HS, et al. 2021. Eneuro. Online ahead of print. PubMed Willuweit A, et al. 2021. Front Neurosci. 15:699926. PubMed Dai Y, et al. 2020. Front Cell Dev Biol. 8:593659. PubMed Song JX, et al. 2020. Aging Cell. 19:e13069. PubMed Li N, et al. 2019. Neural Regen Res. 14:1037. PubMed Magalhães J, et al. 2021. Mol Psychiatry. Online ahead of print. PubMed Arber C, et al. 2020. Mol Psychiatry. 25:2919. PubMed Su Q, et al. 2021. Alzheimers Res Ther. 13:07. PubMed Porter KN, et al. 2020. Front Aging Neurosci. 12:92. PubMed Ye T, et al. 2021. Commun Biol. 4:195. PubMed Ge W, et al. 2021. Am J Transl Res. 13:1471. PubMed Li T, et al. 2016. Nat Commun. 7:12082. PubMed Soto-Mercado V, et al. 2020. PLoS One. 15:e0221669. PubMed Kordower JH, et al. 2017. Ann Neurol. 81:46. PubMed Williamson RL, et al. 2017. J Biol Chem. 292:19873. PubMed Lai RH, et al. 2022. Aging Cell. 21:e13670. PubMed Peng D, et al. 2022. Theranostics. 12:3862. PubMed\u003cbr\u003e\nRRID: AB_2728527 (BioLegend Cat. No. 803014) AB_2565328 (BioLegend Cat. No. 803015) AB_2565329 (BioLegend Cat. No. 803016) AB_2565327 (BioLegend Cat. No. 803017)\u003cbr\u003e\nStructure: Amyloid precursor protein is a 770 amino acid protein with a molecular mass of ~100 kD. According to the UniProtKB database, APP (ID# P05067) has 11 isoforms (34 to ~90 kD) and the 770 form has been designated as the canonical form. Isoform APP695 is the predominant form expressed in neuronal tissue. Isoforms APP751 and APP770 are widely expressed in non-neuronal cells. Isoform APP751 is the most abundant form in T-lymphocytes. Aβ denotes peptides of 36-43 amino acids generated from cleavage of APP by secretases. Aβ has an apparent molecular mass of about 4 kD.\u003cbr\u003e\nDistribution: Tissue distribution: Primarily nervous system, but also adipose tissue, intestine, muscle. Cellular distribution: Cytosol, endosomes, nucleus, plasma membrane, extracellular, and golgi apparatus.\u003cbr\u003e\nFunction: The normal function of Aβ is not well understood. Several potential physiological roles have been proposed, including: activation of kinase enzymes; protection against oxidative stress; regulation of cholesterol transport; transcription factor, and as an anti-microbial agent.\u003cbr\u003e\nBiology Area: Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation\u003cbr\u003e\nMolecular Family: APP\/β-Amyloid\u003cbr\u003e\nAntigen References: Kumar A, et al. 2015. Pharmacol. Rep. 67(2):195. Sadigh-Eteghad S, et al. 2015. Med. Princ. Pract. 24(1):1 Hampel H, et al. 2015. Expert Rev. Neurother. 15(1):83. Puig KL, et al. 2012. Exp. Gerontol. 48(7): 608. Selkoe DJ, et al. 2016. EMBO Mol. Med. 8(6):595. Walsh DM, et al. 2007. J. Neurochem. 101(5):1172.\u003cbr\u003e\nGene ID: 351\u003cbr\u003e\nUniProt: View information about beta-Amyloid 1-16 on UniProt.org\u003cbr\u003e\nClone: 6E10\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: AAA, ABETA, ABPP, AD1, APPI, CTFgamma, CVAP, PN-II, PN2, Amyloid beta A4 protein, preA4, protease, peptidase nexin-II, beta-amyloid peptide, alzheimer disease amyloid protein, cerebral vascular amyloid peptide, APP, Amyloid Precursor Protein\u003cbr\u003e\nPreviously: Signet Catalog# 9300-02Signet Catalog# 9300-05Signet Catalog# 9300-10Covance Catalog# SIG-39300\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862478901417,"sku":"803017","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-803017","provider":"Iright","version":"1.0","type":"link"}