{"product_id":"biolegend-803404","title":"Biolegend, 803404, PE anti-Apo E Antibody, 25tests","description":"\u003cp\u003eApo-E is a class of apolipoprotein primarily synthesized in the liver, but is also found in other tissues such as brain, kidneys, and spleen. Apo-E is a protein component of serum chylomicron and lipoprotein particles, playing essential roles in cholesterol transportation, homeostasis, and metabolism. The expression of Apo-E is induced by cholesterol-rich diets and hyperlipoproteinemia. In the central nervous system, Apo-E is produced by astrocytes and microglia. The Apo-E-containing lipoprotein particles play important functions in brain injury repair, neurogenesis, and synaptic plasticity. Apo-E has been found to be involved in the regulation of amyloid-β aggregation and clearance. Genetic polymorphisms of Apo-E are associated with neuron degenerative disorders including Alzheimer's disease.\u003cbr\u003e\n25tests\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: This antibody was raised against a synthetic peptide corresponding to amino acids surrounding the polymorphic amino acid position 158 of Apo-E.\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: ICFC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication Notes: This antibody is effective in immunoblotting (WB). Clone E6D7 recognizes the E2, E3, and E4 isoforms of apolipoprotein E.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Munoz DG, Feldman H. 2000. CMAJ. 162(1):65-72. Hagberg JM, et al. 2000. Physiol Genomics 4(2):101-108. Saunders AM. 2000. J. Neuropathol. Exp. Neurol. 59(9):751-8. Golabek AA, et al. 2000. Biophys. J. 79(2):1008-15. Zunarelli E, et al. 2000. Breast Cancer Res. Treat. 63(3):193-8. Zunarelli E, et al. 2000. Clin. Neuropathol. 19(1):1-6. Tomiyama T, et al. 1999. Cell Mol. Life Sci. 56(3-4):268-79. Golabek AA., et al. 1999. J. Biol. Chem. 271(18):10602-6.\u003cbr\u003e\nRRID: AB_2801139 (BioLegend Cat. No. 803404) AB_2801140 (BioLegend Cat. No. 803405)\u003cbr\u003e\nCell Type: Astrocytes, Mature Neurons, Microglia, Monocytes, Neurons, Oligodendrocytes\u003cbr\u003e\nBiology Area: Cell Biology, Neurodegeneration, Neuroscience, Neuroscience Cell Markers, Protein Misfolding and Aggregation, Protein Trafficking and Clearance\u003cbr\u003e\nMolecular Family: APP\/Aβ Degradation, Apolipoproteins\u003cbr\u003e\nGene ID: 348\u003cbr\u003e\nUniProt: View information about Apo E on UniProt.org\u003cbr\u003e\nClone: E6D7\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Apolipoprotein E, apolipoprotein E3, AD2, LDLCQ5, LPG, ApoE\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003cbr\u003e\nQ: What type of PE do you use in your conjugates?\u003cbr\u003e\nA: We use R-PE in our conjugates.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46863066464425,"sku":"803404","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-803404","provider":"Iright","version":"1.0","type":"link"}