{"product_id":"biolegend-828801","title":"Biolegend, 828801, Purified anti-PP2AC Methyl (Leu 309) Antibody (Previously Covance catalog# MMS-5117), 100microl","description":"\u003cp\u003ePP2AC (Serine\/threonine-protein phosphatase 2A catalytic subunit alpha isoform), encoded by the PPP2CA gene, is the main phosphatase for microtubule-associated proteins (MAPs). PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. PP2AC is reversibly methyl esterified on Leu-309 by leucine carboxyl methyltransferase 1 (LCMT1) and protein phosphatase methylesterase 1 (PPME1). This carboxyl methylation influences the affinity of the catalytic subunit for the different regulatory subunits, which in turn, modulates the PP2A holoenzyme's enzyme activity and cellular localization.\u003cbr\u003e\n100microl\u003cbr\u003e\nVerified Reactivity: Yeast\u003cbr\u003e\nReported Reactivity: Human, Mouse, Rat\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: This monoclonal antibody was raised against a synthetic peptide from Human methylated Leucine 309 PP2A catalytic subunit.\u003cbr\u003e\nFormulation: Phosphate-buffered solution.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 1 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: This antibody should be handled aseptically as it is free of preservatives such as Sodium Azide. Store this antibody undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check the vial label or CoA to find the proper storage conditions.\u003cbr\u003e\nApplication: WB\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by Western blotting. The optimal working dilution should be determined for each specific assay condition. • WB: 1:100 - 1:800*\u003cbr\u003e\nApplication Notes: This antibody is effective in immunoblotting (WB). * Expected MW: 36 kD WB Positive Control: BY4741 yeast cells The immunogen sequence (C-ßA-RRTPDYFL-Ome) is conserved from human, mouse to yeast.\u003cbr\u003e\nProduct Citations: Padi SKR, et al. 2024. Nature. 195:625. PubMed Vit G, et al. 2022. EMBO J. 41:e110611. PubMed\u003cbr\u003e\nRRID: AB_2564923 (BioLegend Cat. No. 828801)\u003cbr\u003e\nBiology Area: Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation\u003cbr\u003e\nGene ID: 5515\u003cbr\u003e\nUniProt: View information about PP2AC on UniProt.org\u003cbr\u003e\nClone: 2A10\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Serine\/threonine-protein phosphatase 2A catalytic subunit alpha isoform, PP2A-alpha, PPP2CA\u003cbr\u003e\nPreviously: Covance Catalog# MMS-5117\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866550784169,"sku":"828801","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-828801","provider":"Iright","version":"1.0","type":"link"}