{"product_id":"biolegend-841001","title":"Biolegend, 841001, Anti-Neurofilament M (NF-M) Antibody (Previously Covance catalog# PRB-575C), 100microl","description":"\u003cp\u003eNeurofilaments can be defined as the intermediate or 10nm diameter filaments found in neuronal cells. They are composed a mixture of subunits which often includes the neurofilament triplet proteins, NF-L, NF-M and NF-H. Neurofilaments may also include peripherin, a-internexin, nestin and in some cases vimentin. Antibodies in the various neurofilament subunits are very useful cell type markers since the proteins are quite abundant, biochemically stable.\u003cbr\u003e\n100microl\u003cbr\u003e\nVerified Reactivity: Human, Rat, Mouse\u003cbr\u003e\nAntibody Type: Polyclonal\u003cbr\u003e\nHost Species: Rabbit\u003cbr\u003e\nImmunogen: The immunogen was the extreme carboxyterminal region of rat NF-M, which was expressed in E. coli and purified by ion exchange chromatograpy.\u003cbr\u003e\nPreparation: Serum\u003cbr\u003e\nStorage \u0026amp; Handling: Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody.\u003cbr\u003e\nApplication: IHC-P - Quality tested WB - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a dilution range of 1:200 - 1:500 is suggested. For Western blotting, the suggested use of this reagent is 1:5000 - 1:10000. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: The production and characterization of this antiserum similar to monoclonal NF-M clone 3H11. This product may contain other non-IgG subtypes.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Harris J, Ayyub C, Shaw G. A molecular dissection of the carboxyterminal tails of the major neurofilament subunits NF-M and NF-H. J. Neurosci. Res. 30:47-62, 1991.\u003cbr\u003e\nProduct Citations: Vieira JR, et al. 2022. Neuron. 110:4074. PubMed Homma S, et al. 2023. Front Neurosci. 16:1009542. PubMed Min S, et al. 2020. Cell Reports. 29(8):2192-2201.e3.. PubMed Paredes I, et al. 2021. Nat Neurosci. 24:478. PubMed Xu R, et al. 2018. Nat Med. 24:823. PubMed Kirst C, et al. 2020. Cell. 180(4):780-795. PubMed\u003cbr\u003e\nRRID: AB_2565457 (BioLegend Cat. No. 841001)\u003cbr\u003e\nStructure: Expected MW: 145-160 kD\u003cbr\u003e\nCell Type: Mature Neurons\u003cbr\u003e\nBiology Area: Cell Biology, Neuroscience, Neuroscience Cell Markers\u003cbr\u003e\nMolecular Family: Intermediate Filaments\u003cbr\u003e\nGene ID: 4741\u003cbr\u003e\nUniProt: View information about Neurofilament M on UniProt.org\u003cbr\u003e\nClone: Poly28410\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: 160 kD Neurofilament Protein, Neurofilament Triplet M Protein, Neurofilament-3 (150 Kd Medium), Neurofilament, Medium Polypeptide 150kD\u003cbr\u003e\nPreviously: Covance Catalog# PRB-575C\u003cbr\u003e\nIsotype: Rabbit Polyclonal IgG\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866466504873,"sku":"841001","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-841001","provider":"Iright","version":"1.0","type":"link"}