{"product_id":"biolegend-846601","title":"Biolegend, 846601, Purified anti-Tau Phospho (Thr181) Antibody, 25μg","description":"\u003cp\u003eTau protein promotes microtubule assembly and stability. Tau is abundant in neurons of the central nervous system, and is expressed at low levels in astrocytes and oligodendrocytes. Abnormal hyper-phosphorylation, aggregation, and toxic gain of function of tau is associated with several neurological disorders, including Alzheimer’s disease (AD). The major building block of neurofibrillary lesions in AD brains consists of paired helical filaments (PHFs) of abnormally hyperphosphorylated tau. Recent studies indicate that cerebrospinal fluid tau phosphorylated at position threonine 181 has diagnostic utility for several neurological disorders. Six isoforms of tau are generated by alternative splicing of the MAPT gene. These isoforms are distinguished by the number of tubulin binding domains, 3 (3R) or 4 (4R), in the C-terminal of the protein and by one (1N), two (2N), or no (0N) inserts in the N-terminal domain. Tau isoforms are differentially expressed during development.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: 15-mer pT181 Tau peptide conjugated to KLH.\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: IHC-P - Quality testedWB, Direct ELISA - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 10 μg\/ml is suggested. For Western blotting, a concentration range of 2.0 - 10 μg\/ml is suggested. For Direct ELISA, a concentration range of 1.0 - 10 μg\/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nProduct Citations: Rajbanshi B, et al. 2023. Alzheimers Dement. 2874:19. PubMed Kim K, et al. 2020. Nat Commun. 0.540972222. PubMed\u003cbr\u003e\nRRID: AB_2715859 (BioLegend Cat. No. 846601) AB_2616881 (BioLegend Cat. No. 846602)\u003cbr\u003e\nStructure: Unmodified Tau isoforms have an apparent molecular weight ranging from 33-79 kD. Additional high and low molecular weight Tau species have been observed in brain tissues.\u003cbr\u003e\nDistribution: Tissue distribution: central nervous system, peripheral ganglia and nerves, kidney, skeletal, and heart muscle. Cellular distribution: cytoskeleton, nucleus, plasma membrane, and cytosol.\u003cbr\u003e\nFunction: Tau promotes microtubule assembly and stability. The short tau isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.\u003cbr\u003e\nInteraction: Tau interacts with Sequestosome-1, Peptidyl-prolyl cis-trans isomerase FKBP4, Casein kinase I isoform delta, Serine\/threonine-protein kinase Sgk1, Laforin, Alpha-synuclein\u003cbr\u003e\nMolecular Family: Phospho-Proteins, Tau\u003cbr\u003e\nAntigen References: 1. Meredith JE Jr, et al. 2013. PLoS One 8(10):e76523. PubMed 2. Goodall CA, et al. 2006. J. Neurol. Neurosurg. Psychiatry 77(1):89. PubMed\u003cbr\u003e\nGene ID: 4137\u003cbr\u003e\nUniProt: View information about Tau Phospho Thr181 on UniProt.org\u003cbr\u003e\nClone: M7004D06\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Microtubule associated protein tau\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866124341417,"sku":"846601","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-846601","provider":"Iright","version":"1.0","type":"link"}