{"product_id":"biolegend-850506","title":"Biolegend, 850506, Biotin anti-TFAM Antibody, 100μg","description":"\u003cp\u003eTFAM (Transcription Factor A, Mitochondrial) binds to the mitochondrial light strand promoter and functions in mitochondrial transcription regulation, and DNA replication and repair. The protein is required for accurate and efficient promoter recognition by mitochondrial RNA polymerase. Sequence polymorphisms in the TFAM gene are associated with Alzheimer's and Parkinson's diseases. Other diseases associated with TFAM include Mitochondrial DNA Depletion Syndrome, Hepatocerebral Form and Deoxyguanosine Kinase Deficiency.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Full-length human TFAM protein\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.\u003cbr\u003e\nApplication: WB - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 1.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: This antibody recognizes endogenous TFAM in human brain tissues in WB and IHC applications but does not recognize mouse TFAM.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Wang YE, et al. 2013. PLoS One 8(8):e74513. (WB) Caielli S, et al. 2016. J Exp Med. 213(5):697-713. (WB)\u003cbr\u003e\nRRID: AB_2801185 (BioLegend Cat. No. 850505)\u003cbr\u003e\nStructure: TFAM is synthesized as a precursor form with a mitochondrial targeting peptide, which is removed by processing after import to the mitochondria. Mature TFAM has an apparent molecular weight of ~25 kD. Alternative splicing results in multiple transcript variants.\u003cbr\u003e\nDistribution: Tissue distribution: Central nervous system, skin, kidney, lung, thyroid, lymph nodes, breast, esophagus, and heart. Cellular distribution: Mitochondrion, nucleus, and cytosol.\u003cbr\u003e\nFunction: TFAM functions in mitochondrial transcription regulation, and DNA replication and repair.\u003cbr\u003e\nInteraction: TFB1M, TFB2M, CLPX\u003cbr\u003e\nBiology Area: Cell Biology, Mitochondrial Function, Neuroscience, Neuroscience Cell Markers, Transcription Factors\u003cbr\u003e\nMolecular Family: Mitochondrial Markers\u003cbr\u003e\nAntigen References: 1. Bestwick ML, Shadel GS. 2013. Trends Biochem Sci 38(6):283-91. 2. Ramachandran A, et al. 2017. Nucleic Acids Res 45(2):861-874. 3. Günther C, et al. 2004. Neurosci. Lett. 369: 219–223.\u003cbr\u003e\nGene ID: 7019\u003cbr\u003e\nUniProt: View information about TFAM on UniProt.org\u003cbr\u003e\nClone: 18G102B2E11\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Transcription factor A, mitochondrial, mtTFA, MtTF1, TCF6, TCF-6, Transcription Factor 6\u003cbr\u003e\nIsotype: Mouse IgG2b, κ\u003cbr\u003e\nQ: How many biotin molecules are per antibody structure?\u003cbr\u003e\nA: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46860829851817,"sku":"850506","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-850506","provider":"Iright","version":"1.0","type":"link"}