{"product_id":"biolegend-866101","title":"Biolegend, 866101, Purified anti-NRAS Antibody, 25μg","description":"\u003cp\u003eNRAS is a Ras gene family member originally identified in human neuroblastoma cells. Like other members of the Ras family, NRAS has GTP\/GDP binding and GTPase activity, and functions as a G-like regulatory protein that plays a role in regulating cell growth. Mutations in NRAS have been shown to transform cultured cells to tumor cells, implicating its role in tumorigeneses, as potential therapeutic target for cancers such as melanoma.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Human, Mouse, Rat\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Recombinant human NRAS a.a. 1-186\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: WB - Quality testedIHC-P - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. For immunohistochemistry, a concentration range of 2.0 - 10 µg\/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nProduct Citations: Lucas CD, et al. 2022. Sci Immunol. 7:eabm4032. PubMed\u003cbr\u003e\nRRID: AB_2810783 (BioLegend Cat. No. 866101) AB_2810784 (BioLegend Cat. No. 866102)\u003cbr\u003e\nStructure: NRAS is a 189 amino acid protein with predicted and observed molecular mass of ~ 21 kD.\u003cbr\u003e\nDistribution: Tissue Distribution: Ubiquitously expressed. Highly expressed in appendix, gallbladder, and gastrointestinal tract. Cellular Distribution: Plasma membrane, Golgi apparatus, cytosol, and nucleus.\u003cbr\u003e\nFunction: G-Like regulatory protein that plays a role in regulating cell growth.\u003cbr\u003e\nInteraction: RGS14, RASSF7\u003cbr\u003e\nLigand\/Receptor: GTP, GDP\u003cbr\u003e\nCell Type: Astrocytes, Endothelial cells, GABAergic Neurons, Glutamatergic Neurons, Mature Neurons, Microglia, Neural Stem Cells, Oligodendrocytes\u003cbr\u003e\nBiology Area: Cancer Biomarkers, Cell Biology, Cell Proliferation and Viability, Neuroscience, Stem Cells\u003cbr\u003e\nMolecular Family: GTPases\u003cbr\u003e\nAntigen References: Rocks O, et al. 2005. Science. 307:1746-52. Byrne M, Saif MW. 2019. Onco. Targets Ther. S194605 Sexton RE, et al. 2019. Semin. Cancer Biol. 54:131-7.\u003cbr\u003e\nGene ID: 489324605\u003cbr\u003e\nUniProt: View information about NRAS on UniProt.org\u003cbr\u003e\nClone: A18072A\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: NRAS Proto-Oncogene, GTPase NRas, Neuroblastoma RAS Viral Oncogene Homolog\u003cbr\u003e\nIsotype: Mouse IgG2b, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866060640425,"sku":"866101","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-866101","provider":"Iright","version":"1.0","type":"link"}