{"product_id":"biolegend-901505","title":"Biolegend, 901505, Biotin anti-HA.11 Epitope Tag Antibody (Previously Covance catalog# BIOT-101L), 100microl","description":"\u003cp\u003eThe HA tag (hemagglutinin) is an amino acid sequence derived from the human influenza hemagglutinin surface glycoprotein, corresponding to amino acids 98-106.  It is commonly used as a tag to facilitate detection, isolation, and purification of proteins. The full amino acid sequence is: YPYDVPDYA.\u003cbr\u003e\n100microl\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Monoclonal antibody HA.11 was raised against the twelve amino acid peptide CYPYDVPDYASL.\u003cbr\u003e\nFormulation: Phosphate-buffered solution + 50% Glycerol + 0.03% Thimerosal.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 1 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.\u003cbr\u003e\nApplication: WB - Quality tested FC, ICC, IP - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 µg per mL (1:10000 dilution). For immunocytochemistry, a concentration range of 0.5 - 1.0 μg\/mL (1:1000-1:2000 dilution) is recommended. For immunoprecipitation, the suggested use of this reagent is 1:150 dilution. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: Additional tested and reported applications of the 16B12 clone for the relevant formats include: western blot (WB), immunocytochemistry (ICC), immunoprecipitation (IP), and flow cytometry (FC). *Our Posi-Tag Control Protein (931301) can be used as a helpful positive control for this antibody. This second-generation HA antibody is an excellent substitute for the 12CA5 monoclonal antibody. The HA.11 antibody recognizes the influenza hemagglutinin epitope (YPYDVPDYA) which has been used extensively as a general epitope tag in expression vectors. The extreme specificity of the antibody allows unambiguous identification and quantitative analysis of the tagged protein. The HA.11 antibody recognizes HA epitopes located in the middle of protein sequences as well as at the N- or C-terminus.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Kim JY, et al. 2003. J Neurosci. 23:5561. (IP, WB) Helliwell SB, et al. 2001. J Cell Biol. 153:649. (WB) Bennett BD, et al. 2000. J Biol Chem. 275:37712. (IF, IP, WB) Royer Y, et al. 2005. J. Biol. Chem. 29:27251. (FC) Smith BA, et al. 2012. Genes Cancer. 3:550. (IHC) PubMed Hogarth C, et al. 2015. Biol Reprod. 93:19. PubMed Görtz D, et al. 2015. Sci Rep. 5:14685. PubMed Wilson C, et al. 2015. PLoS One. 10:0139579. PubMed Smith B, et al. 2012. Genes Cancer. 3:550-563. PubMed Liu Z, et al. 2016. Nature. 530:98-102. PubMed Thoms M, et al. 2016. Nucleic Acids Res. 44:926-39. PubMed Kim Y, et al. 2016. Nat Commun. 7:10347. PubMed Rodríguez-Escudero M, et al. 2016. PLoS One. 11:0148032. PubMed Lehmann W, et al. 2016. Nat Commun. 7:10498. PubMed Testoni E, et al. 2016. EMBO Mol Med. 8: 105-16. PubMed Padilla S, et al. 2016. Nat Neurosci. 10.1038\/nn.4274. PubMed Martins J, et al. 2016. J Cell Sci. 129:1271-82. PubMed Matak P, et al. 2016. Proc Natl Acad Sci U S A. 113:3428-35. PubMed Starokadomskyy P, et al. 2016. Nat Immunol. 17:495-504. PubMed Mitxelena J, et al. 2016. Nucleic Acids Res. 44:5557-70. PubMed Thongthip S, et al. 2016. Genes Dev. 30:645-59. PubMed Aaes T, et al. 2016. Cell Rep. 15:274-87. PubMed Hodge C, et al. 2016. J Biol Chem. 291:9396-9410. PubMed Alagramam K, et al. 2016. Nat Chem Biol. 12:444-51. PubMed Veit G, et al. 2016. PLoS Biol. 14:1002462. PubMed Lee B, et al. 2016. Development. 143:1721-31. PubMed Douchi D, et al. 2016. Plant Cell. 28:1182-99. PubMed Avgousti D, et al. 2016. Nature. 535:173-77. PubMed Shin H, et al. 2015. Nature. 534:553-7. PubMed Gross G, et al. 2016. Nat Methods. 10.1038\/nmeth.3894. PubMed Dick M, et al. 2016. Nat Commun. 7:11929. PubMed Aldrin-Kirk P, et al. 2016. Neuron. 90:955-68. PubMed Fan R, et al. 2016. Nat Med. 22:780-91. PubMed Rowald K, et al. 2016. Cell Rep. 15:2679-91. PubMed Rampal R, Awasthi A, Ahuja V 2016. Development. 143:2334-43. PubMed Faden F, et al. 2016. Nat Commun. 7:12202. PubMed Lawson C, et al. 2016. Cancer Res. 76: 3826-37. PubMed Szargel R, et al. 2016. Hum Mol Genet. 10.1093\/hmg\/ddw189.. PubMed Damez-Werno D, et al. 2016. Proc Natl Acad Sci U S A. 113: 9623-28. PubMed Su P, et al. 2016. J Immunol. 197: 1054-64. PubMed Morozumi Y, et al. 2016. J Mol Cell Biol. 8:349-62. PubMed Miura Y, et al. 2016. Biochem J. 473:2591-602. PubMed Pashkova N, et al. 2016. Cell Rep. 17:303-15. PubMed Sanchez M, et al. 2016. J Biol Chem. 291:19760-73. PubMed Hwang J, Lee J, Pallas D 2016. J Biol Chem. 291:21008-19. PubMed Jiménez-Canino R, et al. 2016. J Biol Chem. 291:19068-78. PubMed Barquilla A, et al. 2016. Mol Biol Cell. 27:2757-70. PubMed Yadav S, et al. 2016. Sci Rep. 6:34100. PubMed Hashimoto A, et al. 2016. Oncogenesis. 0.388194444. PubMed Guirouilh-Barbat J, et al. 2016. PLoS Genet. 12:e1006230. PubMed Gómez-H L, et al. 2016. Nat Commun. 7:13298. PubMed Rademacher N, et al. 2016. Sci Rep. 6:35283. PubMed Lin Z, et al. 2016. Nat Genet. 10.1038\/ng.3701. PubMed Kaczmarska Z, et al. 2016. Nat Chem Biol. 10.1038\/nchembio.2217. PubMed Zee B, et al. 2016. PLoS One. 11:e0163820. PubMed Despras E, et al. 2016. Nat Commun. 7:13326. PubMed Zhang H, et al. 2016. J Neurosci. 36:11837-50. PubMed Zhang M, et al. 2016. Cell Res. 26:1302-19. PubMed Baehr C, et al. 2016. J Biol Chem. 291:26875-85. PubMed Boehm E, et al. 2016. J Biol Chem. 291:25877-87. PubMed Gail Kilroy, et al. 2016. J Biol Chem. 291:27289-97. PubMed Hongchen Cai, Aimin Liu 2016. Proc Natl Acad Sci U S A. 113:14751-6. PubMed Tam K, et al. 2016. MBio. 7:e02024-16. PubMed Hanke L, et al. 2016. MBio. 7(6). pii: e01569-16. PubMed Stoeber M, et al. 2016. Proc Natl Acad Sci U S A. 113(50):E8069-E8078. PubMed Gu Q, et al. 2016. J Virol. 90:10545-57. PubMed Ramachandran S, et al. 2016. J Biol Chem. 291: 25489-504. PubMed Kaufmann T, et al. 2016. J Cell Sci. 129:4607-21. PubMed Piwko W, et al. 2016. EMBO J. 35:2584-601. PubMed Matsuo Y, et al. 2016. J Cell Sci. 129:4592-606. PubMed Athmer J, et al. 2017. MBio. 10.1128\/mBio.02320-16. PubMed Gao L, et al. 2017. J Cell Sci. 130:396-405. PubMed Gong Y, et al. 2017. Genes Dev. 31:46-58. PubMed K Kataoka, K Mochizuki 2017. J Cell Sci. 130:480-9. PubMed Liszczak G, et al. 2017. Proc Natl Acad Sci U S A. 114:681-6. PubMed Haggie P, et al. 2017. J Biol Chem. 292:771-85. PubMed T Taetzsch, et al. 2017. J Neurosci. 37:70-82. PubMed Yagita Y, et al. 2017. J Biol Chem. 292:691-705. PubMed Chong Jiang, et al. 2017. J Biol Chem. 292:3137-45. PubMed Maeda R, et al. 2017. J Biol Chem. 292:3201-12. PubMed Davis RB, et al. 2017. JCI Insight. 2:e92465. PubMed Daniel JA, et al. 2017. Elife. 6:e26338. PubMed Oishi A, et al. 2017. Sci Rep. 7:8990. PubMed\u003cbr\u003e\nProduct Citations: Hernandez VG, et al. 2023. Glia. 71:1960. PubMed Gregory JA, et al. 2020. Cells. 9:00. PubMed György B, et al. 2017. Mol Metab. 25(2):379-391. PubMed Lambert AR, et al. 2020. Structure. 28(7):760-775. PubMed Motsch V, et al. 2019. Sci Rep. 2.658333333. PubMed Lee EJ, et al. 2022. Sci Rep. 12:8773. PubMed Lee EJ, et al. 2020. Sci Adv. 6:eaay5366. PubMed valos Prado P, et al. 2020. Cell. 184(2):534-544.e11. PubMed Puentes-Mestril C, et al. 2021. J Neurosci. 41:5386. PubMed Trautz B, et al. 2016. J Virol. 90(23):10915-10927. PubMed Li X, et al. 2021. Eur J Immunol. 51:2452. PubMed Kondoh K, et al. 2016. Nature. 532:103-106. PubMed Hanchate NK, et al. 2020. Proc Natl Acad Sci U S A. 117:4375. PubMed\u003cbr\u003e\nRRID: AB_2565023 (BioLegend Cat. No. 901505)\u003cbr\u003e\nBiology Area: Cell Biology\u003cbr\u003e\nGene ID: NA\u003cbr\u003e\nUniProt: View information about HA.11 on UniProt.org\u003cbr\u003e\nClone: 16B12\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: HA epitope tag, HA1, HA2, hemagglutinin, Hemagglutinin HA1 chain, Hemagglutinin HA2 chain, YPYDVPDYA, Hemagglutinin tag\u003cbr\u003e\nPreviously: Covance Catalog# BIOT-101L\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003cbr\u003e\nQ: How many biotin molecules are per antibody structure?\u003cbr\u003e\nA: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46864359063721,"sku":"901505","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-901505","provider":"Iright","version":"1.0","type":"link"}