{"product_id":"biolegend-902001","title":"Biolegend, 902001, Purified anti-AU5 Epitope Tag Antibody (Previously Covance catalog# MMS-135P), 200microl","description":"\u003cp\u003eThe AU5 tag, which consists of the peptide sequence TDFYLK, is often used as a protein modification in order to simplify the labeling and detection of proteins. This unique amino acid sequence allows for specific antibody detection in western blotting and other immunostaining techniques. Due to the short sequence, this modification is not likely to affect the structure or function of the modified proteins.\u003cbr\u003e\n200microl\u003cbr\u003e\nVerified Reactivity: Epitope tag\u003cbr\u003e\nReported Reactivity: Species independent\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: The AU5 monoclonal antibody was raised against an SDS-disrupted BPV-1 preparation and mapped to the six amino acid epitope TDFYLK.\u003cbr\u003e\nFormulation: Phosphate-buffered solution + 0.03% Thimerosal.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 1 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.\u003cbr\u003e\nApplication: WB, ICC, IP, Purification\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by Western blotting. The optimal working dilution should be determined for each specific assay condition. • WB: 1:500* • ICC: 1:500 • IP: 1:150\u003cbr\u003e\nApplication Notes: This antibody is effective in immunofluorescence (IF), immunoblotting, and immunoprecipitation (IP) of tagged proteins. This clone was specifically designed for use in epitope tagging. Because of its small size, clone AU5 is unlikely to alter the activity of the cloned sequence. This antibody was purified using protein-G chromatography.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Burke TW, et al. 2001. J Biol Chem. 276:15397. Marinissen MJ, et al. 2001. Genes Dev. 15:535. Lim PS, et al. 1990. J Infect Dis. 162:1263.\u003cbr\u003e\nProduct Citations: Wroblewska A et al. 2018. Cell. 175(4):1141-1155 . PubMed\u003cbr\u003e\nRRID: AB_2734668 (BioLegend Cat. No. 902007) AB_2565015 (BioLegend Cat. No. 902002)\u003cbr\u003e\nBiology Area: Cell Biology\u003cbr\u003e\nGene ID: NA\u003cbr\u003e\nUniProt: View information about AU5 on UniProt.org\u003cbr\u003e\nClone: AU5\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: AU5 epitope tag, TDFYLK epitope tag, TDFYLK tag\u003cbr\u003e\nPreviously: Covance Catalog# MMS-135P\u003cbr\u003e\nIsotype: Mouse IgG1\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862544076969,"sku":"902001","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-902001","provider":"Iright","version":"1.0","type":"link"}